Klöppinger M, Fertig G, Fraune E, Miltenburger H G
Institute of Zoology, Technical University, Darmstadt, FRG.
Cytotechnology. 1990 Nov;4(3):271-8. doi: 10.1007/BF00563787.
The aim of our study was to establish an efficient system for the in vitro production of the insect pathogenic Autographa californica nuclear polyhedrosis virus in a Spodoptera frugiperda cell line. We optimized cultivation conditions for cell proliferation as well as for virus replication in a 1.5 litre stirred tank bioreactor. Cell and virus propagation were found to be optimal at a constant oxygen tension of 40%. In order to provide sufficient nutrients during virus synthesis filtration and perfusion devices were connected to the bioreactor. A virus production procedure in a repeated batch mode by using a two stage bioreactor system is described. Stage I was optimized for cell production and stage II for virus production.
我们研究的目的是建立一个高效的系统，用于在草地贪夜蛾细胞系中体外生产昆虫病原苜蓿银纹夜蛾核型多角体病毒。我们在1.5升搅拌罐式生物反应器中优化了细胞增殖以及病毒复制的培养条件。发现细胞和病毒增殖在40%的恒定氧张力下最为理想。为了在病毒合成过程中提供足够的营养物质，将过滤和灌注装置连接到生物反应器上。本文描述了一种使用两级生物反应器系统以重复批次模式进行病毒生产的程序。第一阶段针对细胞生产进行了优化，第二阶段针对病毒生产进行了优化。
