用于检测肠道细菌的压电免疫传感器的研制
Development of a piezoelectric immunosensor for the detection of enterobacteria.
作者信息
Plomer M, Guilbault G G, Hock B
机构信息
Department of Botany, Technische Universität München at Weihenstephan, Freising, Germany.
出版信息
Enzyme Microb Technol. 1992 Mar;14(3):230-5. doi: 10.1016/0141-0229(92)90071-u.
PMID:1367979
Abstract
A piezoelectric crystal immunosensor has been developed for the detection of enterobacteria in drinking water using antibodies against the enterobacterial common antigen. Applying an anti-enterobacterial antibody layer via protein A immobilization onto a 10-MHz crystal, a response is observed for 10(6) to 10(9) cells ml-1 of Escherichia coli K12, and for various other antigens of the Enterobacteriaceae family.
摘要
已开发出一种压电晶体免疫传感器，用于使用抗肠杆菌共同抗原的抗体检测饮用水中的肠杆菌。通过蛋白A固定化将抗肠杆菌抗体层应用于10兆赫的晶体上，可观察到对每毫升10⁶至10⁹个细胞的大肠杆菌K12以及肠杆菌科的各种其他抗原产生响应。
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