Zagulski M, Kressler D, Bécam A-M, Rytka J, Herbert C J
Institute of Biochemistry and Biophysics, Polish National Academy of Sciences, 5a Pawinskiego, 02-106 Warsaw, Poland.
Mol Genet Genomics. 2003 Nov;270(3):216-24. doi: 10.1007/s00438-003-0913-4. Epub 2003 Sep 9.
In this study, we show that the Saccharomyces cerevisiae ORF YBR142w, which encodes a putative DEAD-box RNA helicase, corresponds to MAK5. The mak5-1 allele is deficient in the maintenance of the M1 dsRNA virus, resulting in a killer minus phenotype. This allele carries two mutations, G218D in the conserved ATPase A-motif and P618S in a non-conserved region. We have separated these mutations and shown that it is the G218D mutation that is responsible for the killer minus phenotype. Mak5p is an essential nucleolar protein; depletion of the protein leads to a reduction in the level of 60S ribosomal subunits, the appearance of half-mer polysomes, and a delay in production of the mature 25S and 5.8S rRNAs. Thus, Mak5p is involved in the biogenesis of 60S ribosomal subunits.
在本研究中，我们发现酿酒酵母开放阅读框YBR142w编码一种假定的DEAD-box RNA解旋酶，它对应于MAK5。mak5-1等位基因在维持M1双链RNA病毒方面存在缺陷，导致杀伤性阴性表型。该等位基因携带两个突变，保守ATP酶A基序中的G218D和非保守区域中的P618S。我们分离了这些突变，并表明是G218D突变导致了杀伤性阴性表型。Mak5p是一种必需的核仁蛋白；该蛋白的缺失会导致60S核糖体亚基水平降低、半聚核糖体出现以及成熟25S和5.8S rRNA产生延迟。因此，Mak5p参与60S核糖体亚基的生物合成。
