Klein T M, Arentzen R, Lewis P A, Fitzpatrick-McElligott S
DuPont Agricultural Products, Wilmington, DE 19880.
Biotechnology (N Y). 1992 Mar;10(3):286-91. doi: 10.1038/nbt0392-286.
Over the past several years, particle bombardment has evolved into a useful tool for molecular biologists, allowing direct gene transfer to a broad range of cell and tissue types. Some of the important applications of the process include the production of transgenic crop species including maize and soybean and the introduction of DNA into plastids and mitochondria. Recent results have extended the range of gene transfer by particle bombardment to animal and bacterial cells. One noteworthy newer application is the direct insertion of genes into the organs of living animals. Here we discuss these advances and the instrument developments that contributed to them.
在过去几年中，粒子轰击已发展成为分子生物学家的一种有用工具，可将基因直接转移到多种细胞和组织类型中。该技术的一些重要应用包括生产转基因作物品种，如玉米和大豆，以及将DNA导入质体和线粒体。最近的研究结果将粒子轰击的基因转移范围扩展到了动物和细菌细胞。一个值得注意的新应用是将基因直接插入活体动物的器官中。在此，我们将讨论这些进展以及促成这些进展的仪器开发情况。
