Sharma Pushkal, Lew Tedrick Thomas Salim
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, United States.
Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, Singapore.
Front Genome Ed. 2022 Mar 7;4:846624. doi: 10.3389/fgeed.2022.846624. eCollection 2022.
Precise plant genome editing technologies have provided new opportunities to accelerate crop improvement and develop more sustainable agricultural systems. In particular, the prokaryote-derived CRISPR platforms allow precise manipulation of the crop genome, enabling the generation of high-yielding and stress-tolerant crop varieties. Nanotechnology has the potential to catalyze the development of a novel molecular toolbox even further by introducing the possibility of a rapid, universal delivery method to edit the plant genome in a species-independent manner. In this Perspective, we highlight how nanoparticles can help unlock the full potential of CRISPR/Cas technology in targeted manipulation of the plant genome to improve agricultural output. We discuss current challenges hampering progress in nanoparticle-enabled plant gene-editing research and application in the field, and highlight how rational nanoparticle design can overcome them. Finally, we examine the implications of the regulatory frameworks and social acceptance for the future of nano-enabled precision breeding in the developing world.
精确的植物基因组编辑技术为加速作物改良和发展更可持续的农业系统提供了新机遇。特别是，源自原核生物的CRISPR平台允许对作物基因组进行精确操作，从而能够培育出高产和抗逆的作物品种。纳米技术有可能通过引入一种快速、通用的递送方法，以物种独立的方式编辑植物基因组，进一步推动新型分子工具箱的发展。在这篇观点文章中，我们强调了纳米颗粒如何有助于释放CRISPR/Cas技术在靶向操纵植物基因组以提高农业产量方面的全部潜力。我们讨论了当前阻碍基于纳米颗粒的植物基因编辑研究和该领域应用取得进展的挑战，并强调了合理的纳米颗粒设计如何能够克服这些挑战。最后，我们审视了监管框架和社会接受度对发展中世界纳米精准育种未来的影响。
