Puhlmann J, Knaus U, Tubaro L, Schaefer W, Wagner H
Complex Carbohydrate Research Center, Athens, GA 30602.
Phytochemistry. 1992 Aug;31(8):2617-21. doi: 10.1016/0031-9422(92)83597-r.
Two homogeneous, metallic ion-containing pectic polysaccharides with mean M(r)s of 7600 and 15,000 were isolated from dried aerial parts of Achyrocline satureioides by anion exchange column chromatography on DEAE-Sepharose CL-6B and gel filtration column chromatography on Fractogel TSK HW-50 (S). The structures, as determined by methylation analysis, carboxyl reduction, and partial acid hydrolysis, were shown to be rhamnogalacturonans. Both pectins show a pronounced anticomplementary effect in vitro. The larger carbohydrate AS 4 of higher M(r) exerts anti-inflammatory activity and a strong enhancement of phagocytosis in vivo.
通过在DEAE - Sepharose CL - 6B上进行阴离子交换柱色谱和在Fractogel TSK HW - 50 (S)上进行凝胶过滤柱色谱，从牛膝菊的干燥地上部分分离出两种均一的、含金属离子的果胶多糖，其平均相对分子质量分别为7600和15000。通过甲基化分析、羧基还原和部分酸水解确定的结构显示为鼠李半乳糖醛酸聚糖。两种果胶在体外均表现出显著的抗补体作用。相对分子质量较高的较大碳水化合物AS 4在体内具有抗炎活性并能强烈增强吞噬作用。
