费菜的抗炎及免疫活性多糖

Anti-inflammatory and immunologically active polysaccharides of Sedum telephium.

作者信息

Sendl A, Mulinacci N, Vincieri F F, Wagner H

机构信息

Institute of Pharmaceutical Biology, University of Munich, Germany.

出版信息

Phytochemistry. 1993 Nov;34(5):1357-62. doi: 10.1016/0031-9422(91)80029-z.

DOI:10.1016/0031-9422(91)80029-z

PMID:7764285

Abstract

Two major polysaccharides, rhamnogalacturonans with mean M(r)s of 13,500 and 13,000, were isolated from dried leaves of Sedum telephium by column chromatography on DEAE-Sepharose CL-6B and gel filtration on Fractogel TSK HW-50 (S) and Sephacryl 200 HR. The structures were determined mainly by NMR spectroscopy, methylation analysis and GC-MS of the partially methylated alditol acetates, carboxyl reduction and by analysis of acidic and enzymatic degradation products. Both polysaccharides exert an anticomplementary effect in vitro, induce TNF-alpha-production, enhance phagocytosis in vitro and in vivo, and exhibit anti-inflammatory activity.

摘要

通过在DEAE-琼脂糖CL-6B上进行柱色谱以及在Fractogel TSK HW-50 (S) 和Sephacryl 200 HR上进行凝胶过滤，从垂盆草的干燥叶片中分离出两种主要的多糖，即平均分子量分别为13,500和13,000的鼠李半乳糖醛酸聚糖。其结构主要通过核磁共振光谱、甲基化分析以及部分甲基化糖醇乙酸酯的气相色谱-质谱联用、羧基还原反应以及对酸性和酶解降解产物的分析来确定。这两种多糖在体外均具有抗补体作用，可诱导肿瘤坏死因子-α的产生，增强体外和体内的吞噬作用，并表现出抗炎活性。