Puhlmann J, Zenk M H, Wagner H
Institute of Pharmaceutical Biology, University of Munich, F.R.G.
Phytochemistry. 1991;30(4):1141-5. doi: 10.1016/s0031-9422(00)95191-4.
From the nutrition medium of Arnica montana cell cultures two homogeneous polysaccharides, an acidic arabino-3,6-galactan-protein with mean Mr of 100,000 and a neutral fucogalactoxyloglucan with mean Mr of 22,500 have been isolated by DEAE-Sepharose CL-6B and Sephacryl S-400 column chromatography. Their structures were elucidated mainly by methylation analysis, partial acidic and enzymatic hydrolysis and 13C NMR spectroscopy. The fucogalactoxyloglucan shows a pronounced enhancement of phagocytosis in vivo. The arabino-3,6-galactan-protein displays a strong anticomplementary effect and stimulates macrophages to excrete the tumour necrosis factor (TNF alpha).
从山金车属植物细胞培养物的营养培养基中，通过DEAE-琼脂糖CL-6B和Sephacryl S-400柱色谱法分离出两种均一多糖，一种平均分子量为100,000的酸性阿拉伯-3,6-半乳聚糖蛋白和一种平均分子量为22,500的中性岩藻糖基半乳氧基葡聚糖。它们的结构主要通过甲基化分析、部分酸性和酶促水解以及13C NMR光谱进行阐明。岩藻糖基半乳氧基葡聚糖在体内显示出明显的吞噬作用增强。阿拉伯-3,6-半乳聚糖蛋白具有很强的抗补体作用，并刺激巨噬细胞分泌肿瘤坏死因子（TNFα）。
