Pras N
Department of Pharmacognosy, University of Groningen, The Netherlands.
J Biotechnol. 1992 Oct;26(1):29-62. doi: 10.1016/0168-1656(92)90068-k.
The nearly unlimited enzymatic potential of cultured plant cells can basically be employed for bioconversion purposes. Plant enzymes are able to catalyze regio- and stereospecific reactions and can therefore be applied to the production of compounds of pharmaceutical interest. Naturally occurring as well as related synthetic compounds may be used as precursors. A review of the current status of such bioconversions is given. It includes the performance of bioconversions by freely suspended and immobilized plant cells or enzyme preparations. In addition, the kinetic aspects of immobilized plant cells are discussed. Special attention is paid to the bioconversion of poorly or water insoluble precursors. Finally, a model scheme for the development of a commercially available drug, produced by bioconversion, and perspectives are discussed.
培养的植物细胞几乎无限的酶促潜力基本上可用于生物转化目的。植物酶能够催化区域特异性和立体特异性反应，因此可应用于具有药学意义的化合物的生产。天然存在的以及相关的合成化合物都可用作前体。本文对这种生物转化的现状进行了综述。其中包括游离悬浮和固定化的植物细胞或酶制剂进行生物转化的情况。此外，还讨论了固定化植物细胞的动力学方面。特别关注了难溶性或水不溶性前体的生物转化。最后，讨论了通过生物转化生产市售药物的模型方案及前景。
