Guntern R, Bouras C, Hof P R, Vallet P G
University of Geneva, Dept Psychiatry, Switzerland.
Experientia. 1992 Jan 15;48(1):8-10. doi: 10.1007/BF01923594.
Large differences are usually observed when standard staining methods for a number of pathological lesions in neurodegenerative disorders are compared. With the modified thioflavine S method presented here (easy and cheap to perform), the morphological appearance of the stained neurofibrillary tangles (NFT) and senile plaques (SP) is greatly improved. Furthermore, the intense contrast between stained lesions and background obtained with this technique permits an accurate automatic quantification of NFT and SP using a computer-assisted image analysis system.
当比较神经退行性疾病中多种病理病变的标准染色方法时，通常会观察到很大差异。使用本文介绍的改良硫黄素S方法（操作简便且成本低廉），染色的神经原纤维缠结（NFT）和老年斑（SP）的形态外观得到了极大改善。此外，用该技术获得的染色病变与背景之间的强烈对比度允许使用计算机辅助图像分析系统对NFT和SP进行准确的自动定量。
