Dubs M C, Altschuh D, Van Regenmortel M H
Institut de Biologie Moléculaire et Cellulaire, Strasbourg, France.
Immunol Lett. 1992 Jan;31(1):59-64. doi: 10.1016/0165-2478(92)90011-c.
An automated biosensor system designed for measuring molecular interactions in real time and without any labelling of the reactants has been used to study the interaction of two animal viruses (vaccinia virus and poliovirus) and two plant viruses (cowpea mosaic virus and tobacco mosaic virus) with monoclonal antibodies. Using monoclonal antibodies specific for different conformational states of viral protein, it was found that the virus particles retained their conformational integrity when immobilized on the dextran matrix present on the sensor chip. Compared to conventional solid phase immunoassays, in which immobilized proteins are usually partly denatured, the biosensor system presents several advantages for studying virus-antibody interaction.
一种用于实时测量分子相互作用且无需对反应物进行任何标记的自动化生物传感器系统，已被用于研究两种动物病毒（痘苗病毒和脊髓灰质炎病毒）以及两种植物病毒（豇豆花叶病毒和烟草花叶病毒）与单克隆抗体的相互作用。使用针对病毒蛋白不同构象状态的特异性单克隆抗体，发现当病毒颗粒固定在传感器芯片上存在的葡聚糖基质上时，它们保持了构象完整性。与传统的固相免疫测定法相比，在传统方法中固定的蛋白质通常会部分变性，而生物传感器系统在研究病毒 - 抗体相互作用方面具有几个优点。
