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可检测活沙门氏菌的单克隆抗体。

Monoclonal antibodies that detect live salmonellae.

作者信息

Torensma R, Visser M J, Aarsman C J, Poppelier M J, van Beurden R, Fluit A C, Verhoef J

机构信息

Eijkman-Winkler Laboratory for Medical Microbiology, Utrecht, The Netherlands.

出版信息

Appl Environ Microbiol. 1992 Dec;58(12):3868-72. doi: 10.1128/aem.58.12.3868-3872.1992.

DOI:10.1128/aem.58.12.3868-3872.1992
PMID:1476430
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC183196/
Abstract

Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were reactive with serogroups A, B, C1, C2, D, E, and K and Salmonella choleraesuis subsp. diarizonae. Each monoclonal antibody proved to be reactive with a distinct serotype. Clinical isolates belonging to these Salmonella serogroups could be detected. Reactivity with non-Salmonella bacteria proved to be minor.

摘要

通过免疫脾细胞与Sp2/0骨髓瘤细胞的多次融合，获得了9株与活沙门氏菌反应的产生免疫球蛋白G的小鼠单克隆抗体杂交瘤和9株产生免疫球蛋白M的小鼠单克隆抗体杂交瘤。这些抗体通过磁免疫发光测定法进行筛选。单克隆抗体与A、B、C1、C2、D、E和K血清群以及猪霍乱沙门氏菌亚利桑那亚种反应。每株单克隆抗体都与一种独特的血清型反应。属于这些沙门氏菌血清群的临床分离株能够被检测到。与非沙门氏菌的反应较弱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da15/183196/0453587c4474/aem00053-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da15/183196/0453587c4474/aem00053-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da15/183196/0453587c4474/aem00053-0098-a.jpg

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本文引用的文献

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Enhanced hybridoma production by electrofusion in strongly hypo-osmolar solutions.
利用磁免疫聚合酶链反应分析法快速检测家禽中的沙门氏菌。
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Relation between resistance to beta-lactam antibiotics and cadmium in salmonellae isolated from pigs.从猪身上分离出的沙门氏菌中β-内酰胺抗生素耐药性与镉之间的关系。
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Detection of Salmonella species in fecal samples by immunomagnetic separation and PCR.通过免疫磁珠分离和聚合酶链反应检测粪便样本中的沙门氏菌属。
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Outbreak of Salmonella typhimurium infection traced to contaminated chocolate and caused by a strain lacking the 60-megadalton virulence plasmid.鼠伤寒沙门氏菌感染的爆发追溯到受污染的巧克力，由一株缺乏60兆道尔顿毒力质粒的菌株引起。
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