Rafiee P, Ho S B, Bresalier R S, Bloom E J, Kim J H, Kim Y S
Gastrointestinal Research Laboratory, Veterans Administration Medical Center, San Francisco, California 94121.
Pancreas. 1992;7(2):123-31. doi: 10.1097/00006676-199203000-00001.
Cytokeratin-type intermediate filaments are a polygenic family of insoluble proteins that vary according to cell of origin and have been proposed as potentially useful markers of differentiation in epithelial malignancies. Because gastrointestinal malignancies resemble each other in their expression of many soluble antigens, we compared the cytokeratins of seven colonic, three gastric, six pancreatic, and one duodenal carcinoma cell lines, and one colon villous adenoma cell line. Cytokeratins were characterized by one- and two-dimensional gel electrophoresis, immunoblotting, and immunocytochemistry. These cell lines expressed combinations of cytokeratins 7, 8, 18, and 19, which are typical of the "simple" epithelial pattern found in normal ductal and glandular tissues of the gastrointestinal tract. However, pancreatic carcinoma cell lines expressed additional cytokeratins that are normally found in stratified squamous epithelium and epidermoid (squamous cell) carcinomas. These additional cytokeratins consisted of cytokeratin 16 in all six cell lines and cytokeratins 4, 13, and 16 in one cell line. These results suggest that cytokeratin patterns represent stable markers that may aid in distinguishing gastrointestinal malignancies.
细胞角蛋白型中间丝是一类不溶性蛋白质的多基因家族，其根据细胞来源而异，并被认为是上皮性恶性肿瘤中潜在有用的分化标志物。由于胃肠道恶性肿瘤在许多可溶性抗原的表达上彼此相似，我们比较了七种结肠癌细胞系、三种胃癌细胞系、六种胰腺癌细胞系、一种十二指肠癌细胞系以及一种结肠绒毛状腺癌细胞系的细胞角蛋白。通过一维和二维凝胶电泳、免疫印迹和免疫细胞化学对细胞角蛋白进行了表征。这些细胞系表达细胞角蛋白7、8、18和19的组合，这是在胃肠道正常导管和腺组织中发现的“简单”上皮模式的典型特征。然而，胰腺癌细胞系表达了通常在复层鳞状上皮和表皮样（鳞状细胞）癌中发现的其他细胞角蛋白。这些额外的细胞角蛋白在所有六种细胞系中为细胞角蛋白16，在一种细胞系中为细胞角蛋白4、13和16。这些结果表明，细胞角蛋白模式代表了稳定的标志物，可能有助于区分胃肠道恶性肿瘤。
