Lippok B, Brennicke A, Wissinger B
Institut für Genbiologische Forschung, Berlin, FRG.
Mol Gen Genet. 1992 Mar;232(2):322-7. doi: 10.1007/BF00280012.
The gene for cytochrome oxidase subunit II (coxII) in carrot is encoded by a unique locus in the mitochondrial genome. In contrast to the coxII genes in the numerous other plant species investigated to date, the coding region is interrupted by two group II introns. The carrot 5' intron is the homologue of the single intervening sequence found in several monocot and dicot coxII genes. Sequences similar to the 3' intron of the carrot coxII gene have not been reported previously and are not detectable by hybridization with Oenothera mtDNA. Northern hybridizations indicate complex precursor transcript patterns with mRNA molecules up to 10 kb length. The excised intron sequences appear to be stably maintained in the mRNA pool. Amino acid sequence comparisons suggest that the carrot coxII mRNA needs to be edited by numerous C to U transitions.
胡萝卜细胞色素氧化酶亚基II（coxII）基因由线粒体基因组中的一个独特位点编码。与迄今为止研究的许多其他植物物种中的coxII基因不同，其编码区被两个II类内含子打断。胡萝卜的5'内含子是在几个单子叶和双子叶植物coxII基因中发现的单个居间序列的同源物。与胡萝卜coxII基因3'内含子相似的序列以前尚未见报道，用月见草线粒体DNA杂交也检测不到。Northern杂交显示出复杂的前体转录本模式，mRNA分子长度可达10 kb。切除的内含子序列似乎稳定地保留在mRNA库中。氨基酸序列比较表明，胡萝卜coxII mRNA需要通过大量的C到U转换进行编辑。
