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白色念珠菌甘露聚糖中同时含有β-1,2和α-1,2连接的低聚甘露糖残基作为A血清型特异性表位的证据。

Evidence for oligomannosyl residues containing both beta-1,2 and alpha-1,2 linkages as a serotype A-specific epitope(s) in mannans of Candida albicans.

作者信息

Kobayashi H, Shibata N, Suzuki S

机构信息

Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, Miyagi, Japan.

出版信息

Infect Immun. 1992 May;60(5):2106-9. doi: 10.1128/iai.60.5.2106-2109.1992.

Abstract

In order to identify the branches containing both beta-1,2 and alpha-1,2 linkages as the serotype A-specific epitope(s) in the mannans of Candida albicans, serotype A strains with oligosaccharides constituting the beta-1,2 linkage, the alpha-1,2 linkage, and both the beta-1,2 and the alpha-1,2 linkages were prepared from the mannans of C. albicans serotype A strains (NIH A-207 and J-1012) and tested for their inhibitory effects in the precipitin and slide agglutination assays. The results indicated that two oligosaccharides containing both beta-1,2 and alpha-1,2 linkages, Manp beta 1-2Manp alpha 1-2Manp alpha 1-2Manp alpha 1-2Man and Manp beta 1-2Manp beta 1-2Manp alpha 1-2Manp alpha 1-2Manp alpha 1-2Man, served as epitopes participating in the serotype A specificity of C. albicans strains.

摘要

为了鉴定在白色念珠菌甘露聚糖中含有β-1,2和α-1,2连接作为血清型A特异性表位的分支,从白色念珠菌血清型A菌株(NIH A-207和J-1012)的甘露聚糖中制备了具有β-1,2连接、α-1,2连接以及β-1,2和α-1,2连接的寡糖的血清型A菌株,并在沉淀素和玻片凝集试验中测试它们的抑制作用。结果表明,两种含有β-1,2和α-1,2连接的寡糖,即Manpβ1-2Manpα1-2Manpα1-2Manpα1-2Man和Manpβ1-2Manpβ1-2Manpα1-2Manpα1-2Manpα1-2Man,作为参与白色念珠菌菌株血清型A特异性的表位。

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