Serushago B A, Yoshikai Y, Handa T, Mitsuyama M, Muramori K, Nomoto K
Department of Immunology, Kyushu University, Fukuoka, Japan.
Immunology. 1992 Mar;75(3):475-80.
Recombinant human granulocyte colony-stimulating factor (rh G-CSF) enhanced resistance of mice against Listeria monocytogenes (LM) as determined by survival and bacterial growth. Mice pretreated with rh G-CSF twice daily for 5 days survived better than untreated animals to the challenge with LM. Number of bacteria in peritoneal cavity (PC) and spleen was lower in treated mice than that in the control group. Rh G-CSF increased mainly polymorphonuclear cells (PMN) in blood and spleen. After LM inoculation, a larger number of PMN and monocyte-macrophages accumulated in PC and spleen of tested mice. In addition, PMN primed in vivo with rh G-CSF released more superoxide anions when stimulated with phorbol myristate acetate. The inhibition of bacterial growth in PC and spleen could be ascribed to the accumulation of phagocytic cells at the infection sites and the increased oxidative metabolism. The results provided further evidence of the important contribution of G-CSF and neutrophils, as target cells, to the host defence against the intracellular bacteria.
重组人粒细胞集落刺激因子（rh G-CSF）通过小鼠存活情况和细菌生长情况测定，增强了小鼠对单核细胞增生李斯特菌（LM）的抵抗力。每天用rh G-CSF预处理小鼠两次，持续5天，与未处理的动物相比，经LM攻击后存活情况更好。处理组小鼠腹腔（PC）和脾脏中的细菌数量低于对照组。rh G-CSF主要增加了血液和脾脏中的多形核细胞（PMN）。接种LM后，受试小鼠的PC和脾脏中积累了更多的PMN和单核细胞-巨噬细胞。此外，经rh G-CSF体内预处理的PMN在用佛波酯肉豆蔻酸酯刺激时释放更多超氧阴离子。PC和脾脏中细菌生长的抑制可归因于感染部位吞噬细胞的积累和氧化代谢的增加。结果进一步证明了G-CSF和作为靶细胞的中性粒细胞对宿主抵抗细胞内细菌防御的重要贡献。
