Nakagawa S, Takei Y, Bang D, Ueki H
Department of Dermatology, Kawasaki Medical School, Kurashiki, Japan.
Arch Dermatol Res. 1987;279(4):236-40. doi: 10.1007/BF00417321.
Contact sensitivity (CS) induced by hapten has been thought to be analogous to delayed-type hypersensitivity, such as the Mantoux reaction, because of outstanding similarities between the two phenomena. It can be suggested that animals with CS respond also to intradermal injection of the conjugate of hapten and protein as well as to epicutaneous application of hapten. However, evidence against this has been reported. In the present experiments, delayed-type skin reaction (DSR) was successfully obtained in JY1 strain guinea pigs sensitized by painting the skin with 2,4-dinitrochlorobenzene using in vitro dinitrophenylated epidermal cell suspension (DNP-EC) as antigen for a delayed intradermal test. The experiment using anti-Ia alloantiserum and complement showed that the elicitation of DSR is due to the presence of Ia-positive cells (presumably Langerhans cells) among DNP-ECs. The delayed intradermal test with the conjugates such as haptenated ECs in the animals with CS is considered to be an experimentally useful way of analysing the antigen in the sensitivity.
由于这两种现象之间存在显著相似性，半抗原诱导的接触性敏感（CS）被认为类似于迟发型超敏反应，如结核菌素皮试反应。可以推测，患有CS的动物对皮内注射半抗原与蛋白质的结合物以及对表皮涂抹半抗原也会产生反应。然而，已有报道对此提出了反证。在本实验中，通过用2,4-二硝基氯苯涂抹皮肤使JY1品系豚鼠致敏，然后使用体外二硝基苯基化表皮细胞悬液（DNP-EC）作为抗原进行迟发性皮内试验，成功获得了迟发型皮肤反应（DSR）。使用抗Ia同种异体抗血清和补体的实验表明，DSR的引发是由于DNP-EC中存在Ia阳性细胞（可能是朗格汉斯细胞）。对于患有CS的动物，用诸如半抗原化ECs等结合物进行迟发性皮内试验被认为是一种在敏感性分析中对抗原进行实验性分析的有用方法。
