Niculescu F, Rus H G, Retegan M, Vlaicu R
Medical Clinic No. 1, Cluj-Napoca, Romania.
Am J Pathol. 1992 May;140(5):1039-43.
The neoantigens of the C5b-9 complement complex, IgG, C3, C4, S-protein/vitronectin, fibronectin, and macrophages were localized on 17 samples of breast cancer and on 6 samples of benign breast tumors using polyclonal or monoclonal antibodies and the streptavidin-biotin-peroxidase technique. All the tissue samples with carcinoma in each the TNM stages presented C5b-9 deposits on the membranes of tumor cells, thin granules on cell remnants, and diffuse deposits in the necrotic areas. When chemotherapy and radiation therapy preceded surgery, C5b-9 deposits were more intense and extended. The C5b-9 deposits were absent in all the samples with benign lesions. S-protein/vitronectin was present as fibrillar deposits in the connective tissue matrix and as diffuse deposits around the tumor cells, less intense and extended than fibronectin. IgG, C3, and C4 deposits were present only in carcinoma samples. The presence of C5b-9 deposits is indicative of complement activation and its subsequent pathogenetic effects in breast cancer.
使用多克隆或单克隆抗体以及链霉亲和素-生物素-过氧化物酶技术，将C5b-9补体复合物、免疫球蛋白G（IgG）、C3、C4、S蛋白/玻连蛋白、纤连蛋白和巨噬细胞的新抗原定位在17份乳腺癌样本和6份乳腺良性肿瘤样本上。在TNM各期的所有癌组织样本中，肿瘤细胞膜上均出现C5b-9沉积，细胞残骸上有细颗粒，坏死区域有弥漫性沉积。当手术前进行化疗和放疗时，C5b-9沉积更强烈且范围更广。所有良性病变样本中均未出现C5b-9沉积。S蛋白/玻连蛋白以纤维状沉积存在于结缔组织基质中，并以弥漫性沉积存在于肿瘤细胞周围，其强度和范围低于纤连蛋白。IgG、C3和C4沉积仅出现在癌组织样本中。C5b-9沉积的存在表明补体激活及其在乳腺癌中的后续致病作用。
