Lanzer M, de Bruin D, Ravetch J V
DeWitt Wallace Research Laboratory, Division of Molecular Biology, Sloan-Kettering Institute, New York, NY 10021.
EMBO J. 1992 May;11(5):1949-55. doi: 10.1002/j.1460-2075.1992.tb05248.x.
We have mapped Plasmodium falciparum erythrocytic stage transcription units on chromosome 10 in the vicinity of the gene encoding the glycophorin binding protein (GBP130) using yeast artificial chromosomes (YACs). Three erythrocytic stage transcription units are clustered in a 40 kb region. Two of these genes are closely linked, separated by less than 2 kb. Nuclear run-on data demonstrate that transcription of these two genes, though unidirectional, is monocistronic. Within this intergenic region are the sites at which transcription of the upstream gene terminates and the GBP130 gene initiates. These studies represent the first description of the minimal and necessary cis-acting elements for transcription termination and initiation in this protozoan parasite.
我们利用酵母人工染色体（YAC），在编码血型糖蛋白结合蛋白（GBP130）的基因附近，绘制了10号染色体上恶性疟原虫红细胞期转录单位图谱。三个红细胞期转录单位聚集在一个40 kb的区域内。其中两个基因紧密相连，间隔小于2 kb。核转录延伸分析数据表明，这两个基因的转录虽然是单向的，但却是单顺反子的。在这个基因间区域内，存在上游基因转录终止和GBP130基因起始的位点。这些研究首次描述了这种原生动物寄生虫转录终止和起始所需的最小顺式作用元件。
