Rudy B, Kentros C, Weiser M, Fruhling D, Serodio P, Vega-Saenz de Miera E, Ellisman M H, Pollock J A, Baker H
Department of Physiology and Biophysics, New York University Medical Center, New York 10016.
Proc Natl Acad Sci U S A. 1992 May 15;89(10):4603-7. doi: 10.1073/pnas.89.10.4603.
Northern blot analysis and in situ hybridization studies reveal the highly localized expression in rat brain of transcripts from a gene (KShIIIA) encoding components for voltage-gated K+ channels. KShIIIA expression is particularly prominent throughout the dorsal thalamus. The expression of KShIIIA is compared to that of a closely related gene, here called NGK2-KV4. These two genes encode transcripts that induce currents in Xenopus oocytes that are as of yet indistinguishable, but they show very different patterns of expression in rat brain. NGK2-KV4 transcripts are particularly abundant in the cerebellar cortex, where KShIIIA expression is very weak. These results demonstrate the existence of cell-type-specific K+ channel components and suggest that one reason for the unusually large diversity of K+ channel proteins is the presence of subtypes that participate in specific brain functions.
Northern印迹分析和原位杂交研究表明，编码电压门控钾离子通道成分的基因（KShIIIA）在大鼠脑中的转录本具有高度局部化表达。KShIIIA的表达在整个背侧丘脑尤为突出。将KShIIIA的表达与一个密切相关的基因（这里称为NGK2-KV4）的表达进行了比较。这两个基因编码的转录本在非洲爪蟾卵母细胞中诱导出目前尚无法区分的电流，但它们在大鼠脑中显示出非常不同的表达模式。NGK2-KV4转录本在小脑皮质中特别丰富，而KShIIIA在那里的表达非常弱。这些结果证明了细胞类型特异性钾离子通道成分的存在，并表明钾离子通道蛋白异常多样化的一个原因是存在参与特定脑功能的亚型。
