LOCKE M
J Biophys Biochem Cytol. 1961 Aug;10(4):589-618. doi: 10.1083/jcb.10.4.589.
The fine structure and the distribution of an esterase have been studied in the cuticle of Galleria larvae, Tenebrio larvae and pupae, and in the wax-secreting cuticle of the honey bee, and compared with those in the cuticle of the caterpillar of Calpodes. In Galleria and Tenebrio the pore canals are spaces passing through the lamellate endocuticle from the epithelium to the epicuticle. They contain a filament from the cells which may be concerned in their formation. The shape of the pore canal is probably determined by the orientation of the fibres making up the lamellae in the endocuticle and is not a regular helix. The pore canals also contain numerous filaments of another sort which pass on through the epicuticle and are believed to be the origin of the surface wax. They are particularly abundant in the pore canals of the honey bee wax-secreting cuticle and extend into the cell in long pockets surrounded by an envelope of the plasma membrane. The esterase is probably concerned with the final stage of wax synthesis, for its distribution is similar to that of the lipid filaments.
已对大蜡螟幼虫、黄粉虫幼虫和蛹的表皮以及蜜蜂的泌蜡表皮中的一种酯酶的精细结构和分布进行了研究，并与角纹夜蛾幼虫表皮中的酯酶进行了比较。在大蜡螟和黄粉虫中，孔道是从上皮细胞穿过层状内表皮到达上表皮的通道。它们含有来自细胞的细丝，这些细丝可能与孔道的形成有关。孔道的形状可能由构成内表皮薄片的纤维的取向决定，并非规则的螺旋状。孔道中还含有许多其他类型的细丝，这些细丝穿过上表皮，据信是表面蜡的来源。它们在蜜蜂泌蜡表皮的孔道中特别丰富，并以被质膜包膜包围的长袋状延伸到细胞中。酯酶可能与蜡合成的最后阶段有关，因为它的分布与脂质细丝的分布相似。
