Simic M G
Pharmacology and Toxicology Department, School of Pharmacy, University of Maryland, Baltimore 21201.
Mutat Res. 1992 Jun;267(2):277-90. doi: 10.1016/0027-5107(92)90072-a.
Endogenous oxidative processes are shown to generate hydrogen peroxide and .OH radicals, which react in vivo to form a variety of products. Thymidine glycol (Tg) and 8-hydroxydeoxyguanosine (8-dRG-OH) are such products. They result from the excision repair of DNA and are excreted in urine. Both products can be used as biomarkers in the dosimetry of oxidative damage to DNA. Since oxidative processes and accumulation of their effects contribute to carcinogenesis, the proposed rate-of-damage hypothesis provides a rationale for using these biomarkers in early diagnostics and in the assessment of carcinogenic and anticarcinogenic properties of diets, foods, and food components, as well as certain exogenous toxicants and agents. Approaches for measurement of urinary biomarkers of DNA damage are reviewed.
内源性氧化过程可产生过氧化氢和·OH自由基，它们在体内反应形成多种产物。胸苷二醇（Tg）和8-羟基脱氧鸟苷（8-dRG-OH）就是这类产物。它们是DNA切除修复的产物，并通过尿液排出。这两种产物都可作为DNA氧化损伤剂量测定中的生物标志物。由于氧化过程及其效应的积累会导致癌症发生，因此提出的损伤速率假说为在早期诊断以及评估饮食、食物和食物成分以及某些外源性毒物和制剂的致癌和抗癌特性中使用这些生物标志物提供了理论依据。本文综述了测量DNA损伤尿液生物标志物的方法。
