Hellström S, Tengblad A, Johansson C, Hedlund U, Axelsson E
Department of Anatomy, University of Umeå, Sweden.
Histochem J. 1990 Dec;22(12):677-82. doi: 10.1007/BF01047452.
A hyaluronan binding protein (HABP), extracted from cartilage, was biotin-labelled and used for histochemical localization of hyaluronan (HA) in tissue sections. Various tissues were fixed for a mixture of formaldehyde and glutaraldehyde during microwave irradiation. The microwave oven when set at 700 W and 45 degrees C yielded an intense and specific staining of HA. Under these conditions the relative proportion of the two aldehydes did not influence the staining intensity. Aldehyde fixation during microwave irradiation for HA histochemistry, (1) save time, (2) eliminates the use of cetylpyridinium chloride (CPC), and (3) improves the reproducibility.
从软骨中提取的一种透明质酸结合蛋白（HABP）用生物素标记，并用于组织切片中透明质酸（HA）的组织化学定位。在微波照射期间，各种组织用甲醛和戊二醛的混合物固定。微波炉设置为700瓦和45摄氏度时，对HA产生强烈而特异的染色。在这些条件下，两种醛的相对比例不影响染色强度。微波照射期间进行醛固定用于HA组织化学，（1）节省时间，（2）无需使用十六烷基氯化吡啶（CPC），（3）提高了重现性。
