一株T株支原体的尿素水解活性：解脲脲原体。
Urea-hydrolyzing activity of a T-strain mycoplasma: Ureaplasma urealyticum.
作者信息
Masover G K, Sawyer J E, Hayflick L
出版信息
J Bacteriol. 1976 Feb;125(2):581-7. doi: 10.1128/jb.125.2.581-587.1976.
Abstract
The urea-hydrolyzing activity of a T-strain mycoplasma was studied in experiments using whole cells and cell-free enzyme preparations by measuring the release of 14CO2 from [14C]urea. Under the conditions used, the urea concentration optimum is approximately 5.6 X 10(-3) M urea. The activity is soluble and not membrane bound. It is stable at -70 C for several weeks but is more labile at higher temperatures. The pH optimum is between 5.0 and 6.0. The effect of several inhibitors on the activity was tested and revealed similarities, as well as differences, between T-strain mycoplasma urease activity and the urease activity of other organisms and plants.
摘要
通过测量[14C]尿素中14CO2的释放量，在使用全细胞和无细胞酶制剂的实验中研究了T株支原体的尿素水解活性。在所使用的条件下，尿素浓度最佳值约为5.6×10（-3）M尿素。该活性是可溶的，不与膜结合。它在-70℃下可稳定数周，但在较高温度下更不稳定。最适pH值在5.0至6.0之间。测试了几种抑制剂对该活性的影响，结果显示T株支原体脲酶活性与其他生物和植物的脲酶活性之间既有相似之处，也有不同之处。
相似文献
1
Urea-hydrolyzing activity of a T-strain mycoplasma: Ureaplasma urealyticum.
J Bacteriol. 1976 Feb;125(2):581-7. doi: 10.1128/jb.125.2.581-587.1976.
2
Some characteristics of Ureaplasma urealyticum. Urease activity in a simple buffer: effect of metal ions and sulphydryl inhibitors.
J Gen Microbiol. 1978 Oct;108(2):221-5. doi: 10.1099/00221287-108-2-221.
3
Purification and characterization of urease from Ureaplasma urealyticum.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Aug;269(2):160-7. doi: 10.1016/s0176-6724(88)80092-0.
4
T-strain mycoplasmas and genital chlamydiae: inhibition of T-strain urease by hydroxamic acids.
J Infect Dis. 1973 Mar;127:Suppl:S82-3. doi: 10.1093/infdis/127.supplement_1.s82.
5
Localization of enzymes in Ureaplasma urealyticum (T-strain mycoplasma).
J Bacteriol. 1977 Apr;130(1):297-302. doi: 10.1128/jb.130.1.297-302.1977.
6
Effects of carbon dioxide, urea, and ammonia on growth of Ureaplasma urealyticum (T-strain mycoplasma).
J Bacteriol. 1977 Apr;130(1):292-6. doi: 10.1128/jb.130.1.292-296.1977.
7
Growth of T-strain mycoplasmas in medium without added urea: effect of trace amounts of urea and of a urease inhibitor.
J Bacteriol. 1974 Feb;117(2):765-74. doi: 10.1128/jb.117.2.765-774.1974.
8
Hydrolysis of urea by Ureaplasma urealyticum generates a transmembrane potential with resultant ATP synthesis.
J Bacteriol. 1993 Jun;175(11):3253-8. doi: 10.1128/jb.175.11.3253-3258.1993.
9
Dialysis culture of T-strain mycoplasmas.
J Bacteriol. 1974 Apr;118(1):46-52. doi: 10.1128/jb.118.1.46-52.1974.
10
Occurrence of urease in T strains of Mycoplasma.
J Bacteriol. 1967 May;93(5):1513-20. doi: 10.1128/jb.93.5.1513-1520.1967.
引用本文的文献
1
Growth inhibition of Ureaplasma urealyticum by the proton pump inhibitor lansoprazole: direct attribution to inhibition by lansoprazole of urease activity and urea-induced ATP synthesis in U. urealyticum.
Antimicrob Agents Chemother. 1995 Oct;39(10):2187-92. doi: 10.1128/AAC.39.10.2187.
2
Effects of manganese on the growth and morphology of Ureaplasma urealyticum.
J Clin Microbiol. 1984 Jun;19(6):857-64. doi: 10.1128/jcm.19.6.857-864.1984.
3
Characteristics of Ureaplasma urealyticum urease.
J Bacteriol. 1988 Jun;170(6):2692-7. doi: 10.1128/jb.170.6.2692-2697.1988.
4
Effect of urea concentration on growth of Ureaplasma urealyticum (T-strain mycoplasma).
J Bacteriol. 1977 Oct;132(1):144-50. doi: 10.1128/jb.132.1.144-150.1977.
5
Multiple forms of urease in cytoplasmic fractions of Ureaplasma urealyticum.
J Bacteriol. 1977 Jun;130(3):1390-2. doi: 10.1128/jb.130.3.1390-1392.1977.
6
Ureaplasmal epithelial lesions related to ammonia.
Infect Immun. 1977 Mar;15(3):995-6. doi: 10.1128/iai.15.3.995-996.1977.
7
The mycoplasmas.
Microbiol Rev. 1978 Jun;42(2):414-70. doi: 10.1128/mr.42.2.414-470.1978.
8
Effect of ammonium ion concentration and osmotic pressure on growth of Ureaplasma urealyticum (T-strain mycoplasma).
J Bacteriol. 1978 Jun;134(3):967-72. doi: 10.1128/jb.134.3.967-972.1978.
9
Localization of enzymes in Ureaplasma urealyticum (T-strain mycoplasma).
J Bacteriol. 1977 Apr;130(1):297-302. doi: 10.1128/jb.130.1.297-302.1977.
10
Effects of carbon dioxide, urea, and ammonia on growth of Ureaplasma urealyticum (T-strain mycoplasma).
J Bacteriol. 1977 Apr;130(1):292-6. doi: 10.1128/jb.130.1.292-296.1977.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75.
2
UREASE CATALYSIS. II. INHIBITION OF THE ENZYME BY HYDROXYUREA, HYDROXYLAMINE, AND ACETOHYDROXAMIC ACID.
J Biol Chem. 1965 Jun;240:2407-14.
3
UREASE CATALYSIS. I. STOICHIOMETRY, SPECIFICITY, AND KINETICS OF A SECOND SUBSTRATE: HYDROXYUREA.
J Biol Chem. 1965 Jun;240:2402-6.
4
The kinetics of urease activity in Corynebacterium renale.
J Gen Microbiol. 1956 Apr;14(2):478-84. doi: 10.1099/00221287-14-2-478.
5
Purification and properties of bacterial urease.
J Bacteriol. 1954 Jul;68(1):67-73. doi: 10.1128/jb.68.1.67-73.1954.
6
Occurrence of urease in T strains of Mycoplasma.
J Bacteriol. 1967 May;93(5):1513-20. doi: 10.1128/jb.93.5.1513-1520.1967.
7
Influence of urea on the growth of T-strain mycoplasmas.
J Bacteriol. 1967 May;93(5):1509-12. doi: 10.1128/jb.93.5.1509-1512.1967.
8
Proteus vulgaris urease: in vitro inhibition by urea analogues.
Proc Soc Exp Biol Med. 1966 Jun;122(2):506-8. doi: 10.3181/00379727-122-31174.
9
Color test for the measurement of antibody to T-strain mycoplasmas.
J Bacteriol. 1966 Jul;92(1):6-12. doi: 10.1128/jb.92.1.6-12.1966.
10
Tissue cultures and mycoplasmas.
Tex Rep Biol Med. 1965 Jun;23:Suppl 1:285+.
Zotero 插件