al-Bekairi A M, Qureshi S, Ahmed M M, Qazi N S, Khan Z A, Shah A H
Quality Control and Research Laboratory, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Food Chem Toxicol. 1992 Aug;30(8):719-22. doi: 10.1016/0278-6915(92)90169-l.
Treatment with Caralluma tuberculata extract induced complex biochemical and cytological changes in mice. Its cytotoxicity in the bone marrow cells of mice was comparable with that of the standard drug cyclophosphamide (CP); however, unlike CP, C. tuberculata was not clastogenic (as shown by the micronucleus assay). A dose-dependent decrease in the RNA content of liver and testes was produced by C. tuberculata treatment whereas there was no effect on the content of nucleic acid and protein in the brain. In the extract-treated animals there was a significant and dose-dependent increase in the DNA content of the liver, with a negligible effect on the protein content. Combined treatment with C. tuberculata and CP showed that C. tuberculata diminished the effect of CP on DNA levels; however, RNA levels were further suppressed, resulting in increased cytotoxicity. Pretreatment with C. tuberculata extract significantly reduced the clastogenicity of CP. These results indicated the involvement of different phytoconstituents acting by different routes.
用肉质仙人掌提取物处理可诱导小鼠发生复杂的生化和细胞学变化。其对小鼠骨髓细胞的细胞毒性与标准药物环磷酰胺（CP）相当；然而，与CP不同的是，肉质仙人掌没有致断裂作用（微核试验表明）。肉质仙人掌处理可使肝脏和睾丸的RNA含量呈剂量依赖性降低，而对大脑中的核酸和蛋白质含量没有影响。在提取物处理的动物中，肝脏的DNA含量显著且呈剂量依赖性增加，对蛋白质含量的影响可忽略不计。肉质仙人掌与CP联合处理表明，肉质仙人掌可减弱CP对DNA水平的影响；然而，RNA水平进一步受到抑制，导致细胞毒性增加。用肉质仙人掌提取物预处理可显著降低CP的致断裂性。这些结果表明不同的植物成分通过不同途径发挥作用。
