al-Harbi M M, Qureshi S, Ahmed M M, Raza M, Baig M Z, Shah A H
Department of Pharmacology, College of Pharmacy, King Saud University, Saudi Arabia.
J Ethnopharmacol. 1996 Jul 5;52(3):129-37. doi: 10.1016/0378-8741(96)01399-2.
Camel urine treatment was found to cause a significant cytotoxic effect in the bone marrow cells of mice. This cytotoxicity at higher doses was comparable with that of standard drug cyclophosphamide (CP). However, unlike CP, the camel urine treatment failed to induce any clastogenicity. The cytotoxicity induced by camel urine treatment was substantiated by the reduction of liver nucleic acids and glutathione levels and increased malondialdehyde (MDA) contents in the same animals. CP treatment was found to be highly clastogenic, cytotoxic and it reduced the levels of nucleic acids, proteins, glutathione and increased malondialdehyde concentration due to its prooxidant nature. The non-clastogenic nature of camel urine was attributed to the antioxidant and antimutagenic compounds present in camel urine. Pretreatment with camel urine increased the cytotoxicity of CP and intensified the CP induced reduction of liver nucleic acids, glutathione and increased the MDA concentration. The increase of CP induced cytotoxicity appears to be partly due to the additive effect of the two treatments on cellular lipid peroxidation.
研究发现骆驼尿液处理会对小鼠骨髓细胞产生显著的细胞毒性作用。高剂量时这种细胞毒性与标准药物环磷酰胺（CP）相当。然而，与CP不同的是，骆驼尿液处理未诱导任何染色体断裂效应。骆驼尿液处理诱导的细胞毒性可通过同一动物肝脏核酸和谷胱甘肽水平的降低以及丙二醛（MDA）含量的增加得到证实。发现CP处理具有高度的染色体断裂效应、细胞毒性，且由于其促氧化性质，它降低了核酸、蛋白质、谷胱甘肽的水平并增加了丙二醛浓度。骆驼尿液的非染色体断裂性质归因于骆驼尿液中存在的抗氧化和抗诱变化合物。用骆驼尿液预处理会增加CP的细胞毒性，并加剧CP诱导的肝脏核酸、谷胱甘肽的减少以及MDA浓度的增加。CP诱导的细胞毒性增加似乎部分归因于两种处理对细胞脂质过氧化的叠加效应。
