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用丹磺酰氯衍生化后，通过反相柱液相色谱法分离N1-和N8-乙酰亚精胺异构体。

Separation of N1- and N8-acetylspermidine isomers by reversed-phase column liquid chromatography after derivatization with dansyl chloride.

作者信息

Stefanelli C, Carati D, Rossoni C

出版信息

J Chromatogr. 1986 Feb 14;375(1):49-55. doi: 10.1016/s0378-4347(00)83690-7.

DOI:10.1016/s0378-4347(00)83690-7
PMID:3958109
Abstract

The separation of dansyl derivatives of N1- and N8-acetylspermidine by reversed-phase column liquid chromatography is reported. The influence of organic solvents on the retention of acetylspermidines was studied. Best resolutions were achieved using a C18 column and a ternary mobile phase composed of water, methanol and acetonitrile. The precolumn derivatization method permitted the detection of picomole quantities. A method for the determination of acetylspermidines in rat tissues is described.

摘要

报道了通过反相柱液相色谱法分离N1-和N8-乙酰亚精胺的丹磺酰衍生物。研究了有机溶剂对乙酰亚精胺保留的影响。使用C18柱和由水、甲醇和乙腈组成的三元流动相可实现最佳分离效果。柱前衍生化方法可检测皮摩尔量。描述了一种测定大鼠组织中乙酰亚精胺的方法。

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