Uno K, Miyamura K, Kanzaki Y, Fukuda H, Masuyama K, Ishikawa T
Department of Otolaryngology, Kumamoto University School of Medicine, Japan.
Ann Otol Rhinol Laryngol Suppl. 1992 Oct;157:78-81. doi: 10.1177/0003489492101s1016.
Guinea pigs were passively sensitized by sera containing antidinitrophenyl reaginic antibody and specifically challenged by dinitrophenyl-bovine serum albumin injected through the stylomastoid foramen. Nystagmus, head deviation, negative summating potentials on electrocochleography, and an increase of threshold and wave I peak latency on auditory brain stem response testing were observed after local challenge. These physiologic changes were reversible and resolved within several days. We also used Tranilast before the specific challenge. It is a blocking agent of chemical mediator release from mast cells. Negative summating potentials and head deviation were not observed after the use of this agent. In the animals that showed physiologic changes, we observed endolymphatic hydrops, mast cell degranulation, and eosinophil infiltration histologically in the challenged side of the inner ear. These results suggest that the physiologic and histologic changes provoked in the inner ear of the sensitized animals may have been induced by type I allergy.
用含有抗二硝基苯基反应素抗体的血清对豚鼠进行被动致敏，然后通过茎乳孔注射二硝基苯基 - 牛血清白蛋白进行特异性激发。局部激发后，观察到眼震、头部偏斜、耳蜗电图上的负和电位，以及听觉脑干反应测试中阈值升高和I波峰潜伏期延长。这些生理变化是可逆的，在数天内消退。我们还在特异性激发前使用了曲尼司特。它是一种阻止肥大细胞释放化学介质的药物。使用该药物后未观察到负和电位和头部偏斜。在出现生理变化的动物中，我们在内耳激发侧的组织学检查中观察到内淋巴积水、肥大细胞脱颗粒和嗜酸性粒细胞浸润。这些结果表明，致敏动物内耳中引发的生理和组织学变化可能是由I型过敏诱导的。
