Birg F, Courcoul M, Rosnet O, Bardin F, Pébusque M J, Marchetto S, Tabilio A, Mannoni P, Birnbaum D
Laboratory of Molecular Hematology, Unité 119 de l'INSERM, Marseille, France.
Blood. 1992 Nov 15;80(10):2584-93.
FLT3, a receptor belonging to the FMS/KIT family and localized to 13q12, could play a role in the biology of early hematopoietic progenitor cells. Because FMS and KIT are expressed in both normal progenitors and myeloid leukemias, we looked for FLT3 expression in fresh human leukemic cells using Northern blot analysis. High levels of FLT3 expression were detected in 92% of the cases of acute myeloid leukemia (AML) tested, ranging from the M1 to the M5 stages of differentiation assessed in the French-American-British classification. Immature (MO) AML cells, biphenotypic leukemias, and AML with megakaryocytic differentiation (M7 subtype) also expressed the FLT3 transcript. FLT3 was also expressed at high levels in acute lymphoid leukemias of T and B origins. Finally, it was not expressed in chronic myeloid leukemias in chronic phase, whereas it was expressed in most blast crisis samples. This pattern of expression of FLT3 contrasts with the expression of FMS and KIT restricted to myeloid leukemias, and suggests that the FLT3 product could play a role in the expansion of the leukemic blasts of both the myeloid and lymphoid lineages.
FLT3是一种属于FMS/KIT家族的受体，定位于13q12，可能在早期造血祖细胞的生物学过程中发挥作用。由于FMS和KIT在正常祖细胞和髓系白血病中均有表达，我们使用Northern印迹分析在新鲜的人类白血病细胞中寻找FLT3的表达。在92%的检测急性髓系白血病（AML）病例中检测到高水平的FLT3表达，范围从法国-美国-英国分类中评估的M1到M5分化阶段。未成熟（MO）AML细胞、双表型白血病以及具有巨核细胞分化的AML（M7亚型）也表达FLT3转录本。FLT3在T系和B系急性淋巴细胞白血病中也高水平表达。最后，它在慢性期慢性髓系白血病中不表达，而在大多数急变期样本中表达。FLT3的这种表达模式与FMS和KIT仅限于髓系白血病的表达形成对比，并提示FLT3产物可能在髓系和淋巴系白血病母细胞的扩增中发挥作用。
