Rath P M, Rögler G, Schönberg A, Pohle H D, Fehrenbach F J
Abt. für Medizinische Mikrobiologie, Universitätsklinikum Rudolf Virchow, Germany.
Infection. 1992 Sep-Oct;20(5):283-6. doi: 10.1007/BF01710797.
Patients with Borrelia-caused relapsing fever produce cross-reacting antibodies to Borrelia burgdorferi, the anti-genetically related causative agent of Lyme borreliosis. The antibody response of the serum of a patient (acute and convalescent) with relapsing fever was analysed by the immunoblot technique using Borrelia hermsii and B. burgdorferi as antigens. The diagnosis was established by microscopic detection of spirochetes in the patient's blood. The patient's serum showed significantly elevated titers of IgG and IgM in a B. burgdorferi indirect immunofluorescence assay. Immunoblot analysis indicated the presence of cross-reacting antibodies directed to B. burgdorferi antigens with apparent molecular weights of 60, 41, 40, 36, 30 and 20 kDa.
由疏螺旋体引起的回归热患者会产生与伯氏疏螺旋体交叉反应的抗体，伯氏疏螺旋体是莱姆病的抗原相关病原体。采用免疫印迹技术，以赫氏疏螺旋体和伯氏疏螺旋体作为抗原，分析了一名回归热患者（急性期和恢复期）血清的抗体反应。通过显微镜检测患者血液中的螺旋体来确诊。在伯氏疏螺旋体间接免疫荧光试验中，患者血清显示IgG和IgM滴度显著升高。免疫印迹分析表明存在针对伯氏疏螺旋体抗原的交叉反应抗体，这些抗原的表观分子量为60、41、40、36、30和20 kDa。
