Galéa P, Brezinschek R, Lipsky P E, Oppenheimer-Marks N
Harold C. Simmons Arthritis Research Center, University of Texas Southwestern Medical Center, Dallas 75235.
J Immunol. 1994 Jul 15;153(2):529-42.
During inflammation, both CD4+ and CD4- T lymphocytes extravasate into perivascular tissues by adhering to and migrating through the vascular endothelium. These studies were undertaken to characterize the phenotype of CD4- T cells that have a capacity to migrate through endothelium. Results show that CD4- T cells exhibit a greater capacity to migrate through endothelial cells (EC) than CD4+ T cells; and that TCR-gamma delta+ T cells exhibited the greatest migratory capacity. The migrating CD8+ T cell population was enriched in CD45RO+/L-selectin-/LFA-1bright/CD29bright/CD 44bright cells. TNF-alpha-activated EC did not support increased CD4- T cell transendothelial migration and changes in the phenotype of the migrating CD8+ T cells. The migrating CD4- T cell population was enriched in VLA-2+ T cells and expressed increased densities of VLA-4, VLA-5, and VLA-6. The migrating TCR-gamma delta+ T cell population contained both CD8dim and CD8- T cells. Moreover, the migrating gamma delta T cell population was not different from the initial or nonadherent population in that it contained CD45RO+, CD45RA+, and L-selectin+ cells. Finally, migrating TCR-gamma delta+ T cells contained cells expressing V delta 2 and V gamma 9 TCR chains, but these were not enriched compared with the initial population. These studies have characterized the CD4- T cells that are capable of transendothelial migration in vitro. The results are consistent with the conclusion that unique subpopulations of CD8+ alpha beta and CD8+ and CD8- gamma delta T cells gain access to inflammatory sites by virtue of their intrinsic ability to migrate across the endothelium.
在炎症过程中，CD4⁺和CD4⁻ T淋巴细胞通过黏附并穿过血管内皮细胞而渗出到血管周围组织中。进行这些研究是为了表征具有穿过内皮细胞能力的CD4⁻ T细胞的表型。结果显示，CD4⁻ T细胞比CD4⁺ T细胞表现出更强的穿过内皮细胞（EC）的能力；并且TCR-γδ⁺ T细胞表现出最大的迁移能力。迁移的CD8⁺ T细胞群体富含CD45RO⁺/L-选择素⁻/LFA-1bright/CD29bright/CD44bright细胞。肿瘤坏死因子-α激活的内皮细胞不支持CD4⁻ T细胞跨内皮迁移增加以及迁移的CD8⁺ T细胞表型的改变。迁移的CD4⁻ T细胞群体富含VLA-2⁺ T细胞，并表达增加的VLA-4、VLA-5和VLA-6密度。迁移的TCR-γδ⁺ T细胞群体包含CD8dim和CD⁻ T细胞。此外，迁移的γδ T细胞群体与初始或未黏附群体没有差异，因为它包含CD45RO⁺、CD45RA⁺和L-选择素⁺细胞。最后，迁移的TCR-γδ⁺ T细胞包含表达Vδ2和Vγ9 TCR链的细胞，但与初始群体相比，这些细胞并未富集。这些研究表征了能够在体外进行跨内皮迁移的CD4⁻ T细胞。结果与以下结论一致，即CD8⁺αβ和CD8⁺以及CD8⁻γδ T细胞的独特亚群凭借其内在的穿过内皮的能力进入炎症部位。
