Andersen L P, Raskov H, Elsborg L, Holck S, Justesen T, Fischer Hansen B, Møller Nielsen C, Gaarslev K
Dept. Microbiology, Rigshospitalet, University of Copenhagen, Denmark.
APMIS. 1992 Sep;100(9):779-89.
Heat-stable antigens from Helicobacter pylori were investigated for the detection of serum IgG, IgA and IgM antibodies against H. pylori by an ELISA technique. Antibody titers against H. pylori were measured in 167 dyspeptic patients, of whom 96 were H. pylori positive confirmed by culture or microscopy, and in 482 controls (0-98 years). Increased IgG antibody titers were found significantly more often in dyspeptic patients with active chronic gastritis than in patients with normal morphology, as well as in H. pylori-positive patients as compared to H. pylori-negative patients, independent of the endoscopic findings. The heat-stable antigens were compared with acid glycine-extracted antigens and a high degree of concordance was found in the results obtained with the two antigen preparations. The differences in the IgA antibody titers against H. pylori between H. pylori-positive and H. pylori-negative dyspeptic patients were significant and may be useful to confirm a borderline IgG result. No differences were found in IgM antibody titer between H. pylori-positive and -negative patients. The greatest age-dependent increase in IgG and IgA antibody titers was found in children, and if a lower cut-off level is used for children than for adults, as has been proposed, the proportion of people with increased antibody titers against H. pylori would be almost constant from the age of between five and 10 years until the time between 61 and 80 years. Comparison of H. pylori IgG antibodies with IgG antibodies against Campylobacter jejuni and total antibodies against cytomegalovirus (CMV) showed a greater similarity between H. pylori and C. jejuni (R = 0.51) than between H. pylori and CMV (R = 0.22). This may possibly be caused by cross-reactions between H. pylori and C. jejuni. The H. pylori heat-stabile antigen seems not to be very different from other crude H. pylori antigens like acid glycine-extracted antigens, but purification and characterization of the antigens are needed to improve antibody assays.
采用酶联免疫吸附测定（ELISA）技术，对幽门螺杆菌的热稳定抗原进行研究，以检测血清中抗幽门螺杆菌的IgG、IgA和IgM抗体。检测了167例消化不良患者的抗幽门螺杆菌抗体滴度，其中96例经培养或显微镜检查确诊为幽门螺杆菌阳性，还检测了482名对照者（年龄0 - 98岁）的抗体滴度。与胃形态正常的患者相比，活动性慢性胃炎的消化不良患者中，IgG抗体滴度升高的情况更为常见；与幽门螺杆菌阴性患者相比，幽门螺杆菌阳性患者中IgG抗体滴度升高也更为常见，且与内镜检查结果无关。将热稳定抗原与酸甘氨酸提取抗原进行比较，发现两种抗原制剂的检测结果具有高度一致性。幽门螺杆菌阳性和阴性的消化不良患者之间，抗幽门螺杆菌IgA抗体滴度存在显著差异，这可能有助于确认临界的IgG检测结果。幽门螺杆菌阳性和阴性患者之间的IgM抗体滴度未发现差异。儿童中IgG和IgA抗体滴度随年龄增长的增幅最大。如果按照提议，采用比成人更低的临界值水平来检测儿童，那么抗幽门螺杆菌抗体滴度升高的人群比例从5至10岁到61至80岁几乎保持恒定。比较抗幽门螺杆菌IgG抗体与抗空肠弯曲菌IgG抗体以及抗巨细胞病毒（CMV）总抗体发现，幽门螺杆菌与空肠弯曲菌之间的相似性（R = 0.51）高于幽门螺杆菌与CMV之间的相似性（R = 0.22）。这可能是由幽门螺杆菌与空肠弯曲菌之间的交叉反应引起的。幽门螺杆菌热稳定抗原似乎与其他粗制的幽门螺杆菌抗原（如酸甘氨酸提取抗原）没有太大差异，但需要对抗原进行纯化和特性鉴定，以改进抗体检测方法。
