Lecompte F, Castaing M, Pocidalo J J
Bull Eur Physiopathol Respir. 1976 May-Jun;12(3):423-31.
The influence of oxygenation level (oxyhaemoglobin saturation 0 or 100%) on the relationship between plasma pH and erythrocyte pH was studied, in vitro, in normal human blood submitted to changes in carbon dioxide tension. Firstly, the pH of both true and separated erythrolysates were compared: for the former, tonometry was carried out on whole blood, before red cells lysis; for the latter, equilibration was performed on erythrolysate, pH values appeared different: at PCO2 congruent to 21 and 38 Torr, separated erythrolysate was more alkaline than true one, and at PCO2 congruent to 0 it was more acid. Therefore, to estimate pHe-pHi relationship, pHi was evaluated on true erythrolysate. When haemoglobin passed from the reduced to the completely oxygenated state, a significant decrease of both pHe and pHi was observed for a given PCO2 (respectively about 0.05 and 0.08 pH unit), and of pHi for a given pHe (about 0.04 pH unit). In either extra or intraerythrocyte fluid, the oxygen-linked pH difference was negatively correlated to PCO2.
在体外，对暴露于二氧化碳张力变化的正常人血液，研究了氧合水平（氧合血红蛋白饱和度为0或100%）对血浆pH与红细胞pH之间关系的影响。首先，比较了真实红细胞裂解液和分离出的红细胞裂解液的pH：对于前者，在红细胞裂解前对全血进行气体分压测定；对于后者，在红细胞裂解液上进行平衡，pH值出现差异：在二氧化碳分压相当于21和38托时，分离出的红细胞裂解液比真实的更碱性，而在二氧化碳分压相当于0时，它更酸性。因此，为了估计细胞外pH与细胞内pH的关系，在真实红细胞裂解液上评估细胞内pH。当血红蛋白从还原状态转变为完全氧合状态时，对于给定的二氧化碳分压，观察到细胞外pH和细胞内pH均显著降低（分别约为0.05和0.08个pH单位），对于给定的细胞外pH，细胞内pH降低（约0.04个pH单位）。在细胞外或细胞内液中，氧相关的pH差值与二氧化碳分压呈负相关。
