Dlugovitzky D, Luchessi S, Romano B, Molteni O A
Cátedra de Microbiología, Parasitología y Virología, Facultad de Ciencias Médicas, Universidad Nacional de Rosario.
Enferm Infecc Microbiol Clin. 1992 May;10(5):255-8.
To study the effect of tuberculous patients' serum upon normal lymphocytes in order to determine possible inhibition factors of immune response.
We studied total E-rosette formation (4 degrees C, RET) and active rosette formation (37 degrees C, REA) in tuberculous patients in different stages: mild (n:36), moderate (n:28), severe (n:24) and a control group of 32 patients. The same determinations were studied in normal circulating lymphocytes (LN) incubated before with serum from tuberculous patients (SP), serum of normal individuals (SN) and in Tc-199 medium.
The number of RET and REA in tuberculous patients of different stages were significantly lower to the ones of the control group (p less than 0.01, p less than 0.05). This differences were also found when we compare the number of RET and REA after the incubation of LN with SP and SN respectively (p less than 0.01, p less than 0.05).
Tuberculous patients showed an impaired ability to form RET and REA. The incubation of normal lymphocytes with serum from tuberculous patients reduced significantly its capacity to form rosettes.
研究肺结核患者血清对正常淋巴细胞的影响，以确定可能的免疫反应抑制因子。
我们研究了处于不同阶段的肺结核患者（轻度，n = 36；中度，n = 28；重度，n = 24）以及32例患者组成的对照组的总E花环形成率（4℃，RET）和活性花环形成率（37℃，REA）。对正常循环淋巴细胞（LN）分别与肺结核患者血清（SP）、正常人血清（SN）孵育后以及在Tc - 199培养基中孵育后的上述指标进行了同样的测定。
不同阶段肺结核患者的RET和REA数量显著低于对照组（p < 0.01，p < 0.05）。当我们分别比较LN与SP和SN孵育后的RET和REA数量时，也发现了这些差异（p < 0.01，p < 0.05）。
肺结核患者形成RET和REA的能力受损。正常淋巴细胞与肺结核患者血清孵育后，其形成花环的能力显著降低。
