BALDUCCI M, VERANI P, BALDUCCI D
Appl Microbiol. 1963 Jul;11(4):310-4. doi: 10.1128/am.11.4.310-314.1963.
At present there is a lack of standard criteria for the identification and evaluation of activity of antiviral compounds. Interferon was used to explore comparatively several laboratory methods. Interferon was produced in chick embryos and in chorioallantoic membranes suspended in vitro. Evaluation of interferon activity was performed by several methods: (i) percentage of inhibition of plaque-forming units; (ii) hemagglutinin reduction of challenge virus; (iii) titer of cytopathic effect of challenge virus; and (iv) plaque-inhibition test. The suggested methods for measurement are those which express the titer of challenge virus in plaque-forming units or in hemagglutinating units.
目前，在抗病毒化合物活性的鉴定和评估方面缺乏标准准则。使用干扰素对几种实验室方法进行了比较研究。干扰素在鸡胚和体外悬浮的绒毛尿囊膜中产生。通过几种方法评估干扰素活性：（i）空斑形成单位抑制百分比；（ii）攻击病毒血凝素减少量；（iii）攻击病毒细胞病变效应滴度；以及（iv）空斑抑制试验。建议的测量方法是那些以空斑形成单位或血凝单位表示攻击病毒滴度的方法。
