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粘细菌橙色标桩菌和直立标桩菌基因组的大小与稳定性

Size and stability of the genomes of the myxobacteria Stigmatella aurantiaca and Stigmatella erecta.

作者信息

Neumann B, Pospiech A, Schairer H U

机构信息

Zentrum für Molekulare Biologie, University of Heidelberg, Germany.

出版信息

J Bacteriol. 1992 Oct;174(19):6307-10. doi: 10.1128/jb.174.19.6307-6310.1992.

DOI:10.1128/jb.174.19.6307-6310.1992
PMID:1400181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207703/
Abstract

Genomic DNA of Stigmatella aurantiaca DW 4/3.1 was restricted with the rare-cutting endonucleases AseI and SpeI. The restriction pattern derived is composed of 33 AseI and 25 SpeI fragments, whose total size amounts to approximately 9,350 kbp. Genomic fingerprint analysis of chromosomal DNA from several S. aurantiaca isolates further revealed five completely different SpeI and AseI fingerprints and one distinct fingerprint for Stigmatella erecta. In addition, minor variations between the genome sizes of these isolates were observed.

摘要

橙色粘球菌DW 4/3.1的基因组DNA用稀有切割的内切核酸酶AseI和SpeI进行酶切。得到的限制酶切图谱由33个AseI片段和25个SpeI片段组成，其总大小约为9350千碱基对。对几种橙色粘球菌分离株的染色体DNA进行基因组指纹分析，进一步揭示了5种完全不同的SpeI和AseI指纹图谱以及直立粘球菌的一种独特指纹图谱。此外，还观察到这些分离株基因组大小之间的微小差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb2d/207703/1699bcbf5ca7/jbacter00085-0319-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb2d/207703/699c0a65ab43/jbacter00085-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb2d/207703/183697e7ce3a/jbacter00085-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb2d/207703/1699bcbf5ca7/jbacter00085-0319-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb2d/207703/699c0a65ab43/jbacter00085-0317-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb2d/207703/183697e7ce3a/jbacter00085-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb2d/207703/1699bcbf5ca7/jbacter00085-0319-a.jpg

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