黄色粘球菌染色体的物理图谱。
A physical map of the Myxococcus xanthus chromosome.
作者信息
He Q, Chen H, Kuspa A, Cheng Y, Kaiser D, Shimkets L J
机构信息
Department of Microbiology, University of Georgia, Athens 30602.
出版信息
Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9584-7. doi: 10.1073/pnas.91.20.9584.
A physical map of the 9.2-Mbp Myxococcus xanthus DK1622 chromosome at a resolution of 25 kbp was constructed by using a strategy that is applicable to virtually all microorganisms. Segments of the chromosome were used as hybridization probes to subdivide a yeast artificial chromosome (YAC) library into groups of linked clones. The clones were aligned by comparing their EcoRI restriction patterns. The groups of YAC clones ("contigs") were oriented and aligned with the genomic restriction map by means of common genetic and physical markers such as rare restriction sites and transposon insertions. Over 95% of the genome is represented by cloned DNA. Sixty genetic loci including > 100 genes, many of which play a role in fruiting body development, have been mapped in this way. Additional genes can now be located on the chromosome map by hybridization of their sequences to the ordered set of YAC chromosomes. The mapped genetic loci account for approximately 2% of the genome.
采用一种几乎适用于所有微生物的策略，构建了分辨率为25 kbp的9.2 Mbp黄色黏球菌DK1622染色体物理图谱。染色体片段用作杂交探针，将酵母人工染色体（YAC）文库细分为连锁克隆群。通过比较它们的EcoRI限制性酶切图谱来排列克隆。借助稀有限制性酶切位点和转座子插入等常见遗传和物理标记，将YAC克隆群（“重叠群”）进行定向并与基因组限制性图谱对齐。超过95%的基因组由克隆DNA代表。通过这种方式已定位了60个遗传位点，包括100多个基因，其中许多基因在子实体发育中起作用。现在可以通过将其他基因的序列与有序的YAC染色体组杂交，将它们定位在染色体图谱上。已定位的遗传位点约占基因组的2%。
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