WATANABE T, OGATA C, SATO S
J Bacteriol. 1964 Oct;88(4):922-8. doi: 10.1128/jb.88.4.922-928.1964.
Watanabe, Tsutomu (Keio University School of Medicine, Tokyo, Japan), Chizuko Ogata, and Sachiko Sato. Episome-mediated transfer of drug resistance in Enterobacteriaceae. VIII. Six-drug-resistance R factor. J. Bacteriol. 88:922-928. 1964.-The multiple-drug-resistant Escherichia coli strain isolated by Lebek in 1963 was found to transfer resistance to sulfonamide, streptomycin, chloramphenicol, tetracycline, kanamycin, and neomycin together by conjugation, as well as by transduction with phage P1kc, suggesting that these drug-resistance markers are carried by a single R factor (R(6)). The results of transductional and spontaneous segregations of the drug-resistance markers of R(6) have shown that R(6) has independent genetic determinants for sulfonamide, streptomycin, chloramphenicol, tetracycline, and kanamycin-neomycin resistance. Resistance to kanamycin and neomycin is probably controlled by a single gene, because no segregation was observed between these two. The resistance transfer factor of R(6) was found to be of the fi(+) type.
渡边勉(日本东京庆应义塾大学医学院)、绪方千鹤子和佐藤幸子。肠杆菌科中附加体介导的耐药性转移。VIII. 六药耐药R因子。《细菌学杂志》88:922 - 928,1964年。——1963年莱贝克分离出的多重耐药大肠杆菌菌株被发现可通过接合作用以及用噬菌体P1kc进行转导,将对磺胺类药物、链霉素、氯霉素、四环素、卡那霉素和新霉素的耐药性一起转移,这表明这些耐药性标记由单个R因子(R(6))携带。R(6)耐药性标记的转导和自发分离结果表明,R(6)具有磺胺类药物、链霉素、氯霉素、四环素以及卡那霉素 - 新霉素耐药性的独立遗传决定因素。对卡那霉素和新霉素的耐药性可能由单个基因控制,因为在这两者之间未观察到分离现象。发现R(6)的耐药性转移因子为fi(+)型。