Crabtree J E, Shallcross T M, Heatley R V, Wyatt J I
Department of Medicine, St James's University Hospital, Leeds.
J Clin Pathol. 1991 Apr;44(4):326-8. doi: 10.1136/jcp.44.4.326.
A commercial ELISA for the detection of Helicobacter pylori IgG antibodies was evaluated using serum from 242 patients attending an endoscopy clinic. The efficacy of the ELISA was assessed in relation to the histological detection of H pylori on antral mucosal biopsy specimens. In patients under 61 years of age (n = 138) the ELISA was 97.5% sensitive and 85.5% specific for H pylori infection, with a positive predictive value of 91% and a negative predictive value of 96%. Over the whole group the sensitivity of the ELISA was 93.8% and the specificity 79.3%. The positive predictive value and negative predictive values were, respectively, 90% and 87%. These results suggest that the Bio-Rad GAP IgG H pylori ELISA is suitable for serodiagnosis of H pylori infections for most clinical purposes and thus makes H pylori serology available to routine diagnostic laboratories.
使用来自一家内镜检查诊所的242例患者的血清，对一种用于检测幽门螺杆菌IgG抗体的商用酶联免疫吸附测定（ELISA）进行了评估。根据胃窦黏膜活检标本中幽门螺杆菌的组织学检测结果，评估了ELISA的有效性。在61岁以下的患者（n = 138）中，ELISA对幽门螺杆菌感染的敏感性为97.5%，特异性为85.5%，阳性预测值为91%，阴性预测值为96%。在整个研究组中，ELISA的敏感性为93.8%，特异性为79.3%。阳性预测值和阴性预测值分别为90%和87%。这些结果表明，Bio-Rad GAP IgG幽门螺杆菌ELISA适用于大多数临床目的的幽门螺杆菌感染血清学诊断，从而使常规诊断实验室能够开展幽门螺杆菌血清学检测。
