Hirschl A M, Pletschette M, Hirschl M H, Berger J, Stanek G, Rotter M L
Hygiene-Institute, University of Vienna, Austria.
Eur J Clin Microbiol Infect Dis. 1988 Aug;7(4):570-5. doi: 10.1007/BF01962618.
This study presents a novel approach to the analysis of protein antigens of Campylobacter pylori for use in serology. Protein fractions of this bacterium were resolved in polyacrylamide gel electrophoresis, eluted from gel strips in an electric field and used for coating of microtiter plates in an ELISA-type assay run with a small set of sera from both infected and non-infected patients. Reactivity and discriminative power of the different fractionated antigens (1-9) and crude antigen preparations (A-C) were compared. Better discrimination was achieved between positive and negative sera with high molecular weight fractionated preparations (antigens 8 and 9) than with low molecular weight fractions. Among the crude antigen preparations, antigens A (sonicated whole cells) and C (ultracentrifugated sonicate) seem to have a better discriminative power than antigen B (acid glycin extract).
本研究提出了一种用于血清学分析幽门螺杆菌蛋白质抗原的新方法。该细菌的蛋白质组分在聚丙烯酰胺凝胶电泳中进行分离，在电场中从凝胶条带洗脱，并用于在酶联免疫吸附测定（ELISA）类型的检测中包被微量滴定板，该检测使用了一小部分来自感染和未感染患者的血清。比较了不同分级抗原（1 - 9）和粗抗原制剂（A - C）的反应性和鉴别能力。与低分子量级分相比，高分子量级分制剂（抗原8和9）在阳性和阴性血清之间实现了更好的区分。在粗抗原制剂中，抗原A（超声处理的全细胞）和抗原C（超速离心超声提取物）似乎比抗原B（酸性甘氨酸提取物）具有更好的鉴别能力。
