Best L M, Veldhuyzen van Zanten S J, Sherman P M, Bezanson G S
Department of Microbiology, Victoria General Hospital, Halifax, Nova Scotia, Canada.
J Clin Microbiol. 1994 May;32(5):1193-6. doi: 10.1128/jcm.32.5.1193-1196.1994.
The antibody response to Helicobacter pylori was examined in 56 children (ages 5 to 18) to determine whether serological tests can be used for diagnosis. Twenty-four children (43%) were H. pylori positive and 32 children (57%) were H. pylori negative by culture and histological examination of endoscopic biopsy specimens. The immune response was also examined in 39 nonendoscoped parents of the children. H. pylori-specific immunoglobulin G (IgG) and IgA antibodies were detected by the flow microsphere immunofluorescent assay (FMIA). IgG was also detected by using the Pyloristat enzyme-linked immunosorbent assay (ELISA). The sensitivity, specificity, and positive and negative predictive values for the FMIA for IgG were 100, 97, 96, and 100%, respectively. The respective values for the Pyloristat ELISA for IgG were 96, 94, 92, and 97%. The respective values for the FMIA for IgA were 50, 100, 100, and 73%. Both assays identified the same 19 parents as IgG positive, while FMIA identified 17 of the 19 parents as IgA positive.
对56名儿童（5至18岁）的幽门螺杆菌抗体反应进行了检测，以确定血清学检测是否可用于诊断。通过对内镜活检标本进行培养和组织学检查，24名儿童（43%）幽门螺杆菌呈阳性，32名儿童（57%）幽门螺杆菌呈阴性。还对39名未接受内镜检查的儿童父母的免疫反应进行了检测。采用流式微球免疫荧光测定法（FMIA）检测幽门螺杆菌特异性免疫球蛋白G（IgG）和IgA抗体。还使用幽门螺旋杆菌酶联免疫吸附测定法（ELISA）检测IgG。FMIA检测IgG的敏感性、特异性、阳性预测值和阴性预测值分别为100%、97%、96%和100%。幽门螺旋杆菌ELISA检测IgG的相应值分别为96%、94%、92%和97%。FMIA检测IgA的相应值分别为50%、100%、100%和73%。两种检测方法均将相同的19名父母鉴定为IgG阳性，而FMIA将这19名父母中的17名鉴定为IgA阳性。
