用于幽门螺杆菌低温长期保存的选定冷冻保护剂。
Selected cryopreservatives for long term storage of Helicobacter pylori at low temperatures.
作者信息
Shahamat M, Paszko-Kolva C, Mai U E, Yamamoto H, Colwell R R
机构信息
Department of Microbiology, University of Maryland, College Park 20742.
出版信息
J Clin Pathol. 1992 Aug;45(8):735-6. doi: 10.1136/jcp.45.8.735.
Abstract
To meet the need for information on cryopreservation, a study was done on 32 Helicobacter pylori strains, comparing different cryopreservative media. Sheep blood, horse blood, horse serum with and without glycerol, and mineral oil media were used for long term storage of H pylori at -70 degrees C or in liquid nitrogen. Procedures were developed which permitted recovery of 87.5% of the strains included in the study after they had been stored for 24 months. Of those strains stored for more than three years, 60% were recovered. It is concluded that most strains of H pylori can be stored for up to one year or longer, under refrigeration, at -70 degrees C or in liquid nitrogen.
摘要
为满足对冷冻保存信息的需求，对32株幽门螺杆菌菌株进行了一项研究，比较不同的冷冻保存培养基。使用绵羊血、马血、含甘油和不含甘油的马血清以及矿物油培养基在-70℃或液氮中对幽门螺杆菌进行长期保存。开发了相应程序，使研究中87.5%的菌株在储存24个月后能够复苏。在储存超过三年的菌株中，60%能够复苏。结论是，大多数幽门螺杆菌菌株在冷藏、-70℃或液氮条件下可保存长达一年或更长时间。
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