从婴儿猝死综合征分离出的细菌毒素的致死协同作用。
Lethal synergistic action of toxins of bacteria isolated from sudden infant death syndrome.
作者信息
Drucker D B, Aluyi H S, Morris J A, Telford D R, Gibbs A
机构信息
Department of Cell and Structural Biology, University of Manchester.
出版信息
J Clin Pathol. 1992 Sep;45(9):799-801. doi: 10.1136/jcp.45.9.799.
AIM
To test the hypothesis that lethal toxins of bacteria associated with sudden infant death syndrome (SIDS) can act synergistically.
METHODS
Bacteria occurring together in the nasopharynx of cases of cot death were studied. The lethal toxicity of crude toxin preparations was determined over a range of dilutions by injections into the chorioallantoic vein of the chick embryo. Toxin preparations of low lethality for the chick embryo SIDS model were then tested in combination.
RESULTS
Staphylococcus aureus toxin preparations showed low lethality when tested alone, even at low dilution. At 1 in 100 dilution S aureus toxin was lethal to one out of 15 chick embryos. Escherichia coli toxin preparations showed high lethality except on high dilution (1 in 80) when lethality fell to two out of 15 of chick embryos. When the same toxin preparations were tested simultaneously in combination, lethality rose to 14 out of 15. Similar findings were observed over a range of toxin dilutions. This finding was highly significant (p = 0.0012).
CONCLUSIONS
That synergy between toxins can enhance the lethality of toxins elaborated by bacteria associated with SIDS.
目的
验证与婴儿猝死综合征（SIDS）相关的细菌致死毒素具有协同作用这一假说。
方法
对共死于婴儿床的病例鼻咽部共生的细菌进行研究。通过向鸡胚绒毛尿囊静脉注射一系列不同稀释度的粗毒素制剂来测定其致死毒性。然后对鸡胚SIDS模型致死率低的毒素制剂进行联合测试。
结果
单独测试时，金黄色葡萄球菌毒素制剂即使在低稀释度下也显示出低致死率。在100倍稀释时，金黄色葡萄球菌毒素对15只鸡胚中的1只具有致死性。大肠杆菌毒素制剂除了在高稀释度（80倍）时致死率降至15只鸡胚中的2只外，显示出高致死率。当同时对相同的毒素制剂进行联合测试时，致死率升至15只鸡胚中的14只。在一系列毒素稀释度下均观察到类似结果。这一发现具有高度显著性（p = 0.0012）。
结论
毒素之间的协同作用可增强与SIDS相关细菌所产生毒素的致死性。
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