Yamazaki T
Department of Pathobiology, University of Washington.
Kansenshogaku Zasshi. 1992 May;66(5):632-6. doi: 10.11150/kansenshogakuzasshi1970.66.632.
A strain of Chlamydia pneumoniae (C. pneumoniae) which had been isolated from a Japanese child (AC-43) was examined morphologically and serologically using micro-immunofluorescent (micro-IF) test. Inclusions of AC-43 were stained by an indirect immunofluorescent method using C. pneumoniae specific monoclonal antibody. They were dense round inclusions which had been reported as a characteristic figure for C. pneumoniae. An elementary body (EB) of AC-43 was pear-shaped by electron micrograph, which was the same as previous reports for C. pneumoniae. We produced monoclonal antibodies using purified EB of AC-43 as antigen. Culture fluids of these clones reacted with C. pneumoniae antigens, but did not react with C. trachomatis or C. psittaci antigens by micro-IF tests. There was no difference in morphological and serological findings among standard strains and Japanese isolate of C. pneumoniae.
对从一名日本儿童分离出的一株肺炎衣原体（C. pneumoniae）（AC - 43）进行了形态学和血清学检查，采用微量免疫荧光（micro - IF）试验。使用肺炎衣原体特异性单克隆抗体通过间接免疫荧光法对AC - 43的包涵体进行染色。它们是致密的圆形包涵体，这是肺炎衣原体的特征性形态。通过电子显微镜观察，AC - 43的原体（EB）呈梨形，这与先前关于肺炎衣原体的报道一致。我们以纯化的AC - 43的EB作为抗原制备单克隆抗体。这些克隆的培养液与肺炎衣原体抗原发生反应，但通过微量免疫荧光试验不与沙眼衣原体或鹦鹉热衣原体抗原发生反应。肺炎衣原体的标准菌株和日本分离株在形态学和血清学结果上没有差异。
