Miyashita N, Matsumoto A, Soejima R, Kubota Y, Kishimoto T, Nakajima M, Niki Y, Matsushima T
Department of Medicine, Kawasaki Medical School, Japan.
Kansenshogaku Zasshi. 1996 Mar;70(3):224-31. doi: 10.11150/kansenshogakuzasshi1970.70.224.
We examined the utility of the direct immunofluorescent antibody test kit, IMAGEN (DAKO Diagnostic Co., Ltd.). The stainability of inclusions and purified elementary bodies (EBs) of all the Chlamydia pneumoniae strains used in this study was highly specific. Immunoelectron microscopy and light microscopy of stained EBs revealed the target antigen of IMAGEN to be located on the surface of the EB outer membrane. In a clinical study, we tested oropharyngeal swab specimens obtained from 41 patients (45 specimens). The results were compared with those obtained by isolation in cell culture, the indirect immunofluorescent antibody (IFA) assay and serum antibody titration. C. pneumoniae was isolated from six specimens (13.3%), and the organisms were detected in 11 specimens (24.4%) with IMAGEN and nine specimens (20%) with IFA. A diagnosis of C. pneumoniae infection was made in six patients (13.3%) by the serological examination. Six specimens positive in isolation were positive in the IMAGEN but there were three cases which seemed to be false-positive by the IMAGEN. We conclude that the use of IMAGEN is an easy, rapid and sensitive method for detecting C. pneumoniae when there is a large amount of chlamydial antigen in the clinical specimen and when identification of the chlamydial species forming inclusions in cell cultures is required.
我们检测了直接免疫荧光抗体检测试剂盒IMAGEN（达科诊断有限公司）的效用。本研究中所用的所有肺炎衣原体菌株的包涵体和纯化原体（EB）的染色性具有高度特异性。对染色后的EB进行免疫电子显微镜和光学显微镜检查发现，IMAGEN的靶抗原位于EB外膜表面。在一项临床研究中，我们检测了从41例患者（45份标本）获取的口咽拭子标本。将结果与通过细胞培养分离、间接免疫荧光抗体（IFA）检测和血清抗体滴定获得的结果进行比较。从6份标本（13.3%）中分离出肺炎衣原体，通过IMAGEN在11份标本（24.4%）中检测到该病原体，通过IFA在9份标本（20%）中检测到。通过血清学检查，6例患者（13.3%）被诊断为肺炎衣原体感染。6份分离阳性的标本在IMAGEN检测中呈阳性，但有3例在IMAGEN检测中似乎为假阳性。我们得出结论，当临床标本中有大量衣原体抗原且需要鉴定在细胞培养中形成包涵体的衣原体种类时，使用IMAGEN是一种检测肺炎衣原体的简便、快速且灵敏的方法。
