Carlson J, Wahlgren M
Department of Immunology, Stockholm University, Sweden.
J Exp Med. 1992 Nov 1;176(5):1311-7. doi: 10.1084/jem.176.5.1311.
Herein we describe an assay that was developed to quantitate the binding of normal red blood cells (RBC), labeled with carboxy fluorescein diacetate (C-FDA), to rosetting Plasmodium falciparum-infected RBC. The binding of RBC obtained from various animal species or humans to different strains or clones of rosetting P. falciparum-infected RBC was studied. A strain-specific preference of rosetting was observed for either blood group A/AB or B/AB RBC for all parasites tested. The higher affinity of rosette binding of blood group A, B, or AB vs. O RBC was reflected in larger rosettes when a given parasite was grown in RBC of the preferred blood group. The small size of the rosettes formed when P. falciparum was grown in blood group O RBC may be the in vitro correlate of the relative protection against cerebral malaria afforded by belonging to blood group O rather than to blood group A or B. Rosettes of a blood group A-preferring parasite could be completely disrupted by heparin only when grown in blood group O or B RBC, but not when grown in blood group A RBC. Similarly, the rosettes of a blood group B-preferring parasite could be more easily disrupted by heparin when grown in blood group O or A RBC than when grown in blood group B RBC. Several different saccharides inhibited rosetting of group O RBC, including two monosaccharides that are basic components of heparin. The rosetting of the same parasites grown in blood group A or B RBC was less sensitive to heparin and was specifically inhibited only by the terminal mono- and trisaccharides of the A and the B blood group antigens, the H disaccharide, and fucose. Our results suggest that rosetting is mediated by multiple lectin-like interactions, the usage of which rely on the parasite phenotype and whether the receptors are present on the host cell or not.
在此，我们描述了一种检测方法，该方法用于定量标记有羧基荧光素二乙酸酯（C-FDA）的正常红细胞（RBC）与形成玫瑰花结的恶性疟原虫感染的红细胞的结合。研究了从各种动物物种或人类获得的红细胞与不同菌株或克隆的形成玫瑰花结的恶性疟原虫感染的红细胞的结合情况。对于所有测试的寄生虫，观察到A/AB或B/AB血型红细胞对玫瑰花结形成具有菌株特异性偏好。当给定的寄生虫在偏好血型的红细胞中生长时，A、B或AB血型红细胞与O型红细胞相比，玫瑰花结结合的亲和力更高，这表现为更大的玫瑰花结。当恶性疟原虫在O型血红细胞中生长时形成的玫瑰花结较小，这可能是在体外表明O型血相对于A或B型血对脑型疟疾具有相对保护作用的关联因素。仅当偏好A型血的寄生虫在O型或B型血红细胞中生长时，其玫瑰花结才能被肝素完全破坏，而在A型血红细胞中生长时则不能。同样，偏好B型血的寄生虫的玫瑰花结在O型或A型血红细胞中生长时比在B型血红细胞中生长时更容易被肝素破坏。几种不同的糖类可抑制O型血红细胞的玫瑰花结形成，包括肝素的两种单糖基本成分。在A型或B型血红细胞中生长的相同寄生虫的玫瑰花结对肝素的敏感性较低，并且仅被A和B血型抗原的末端单糖和三糖、H二糖和岩藻糖特异性抑制。我们的结果表明，玫瑰花结形成是由多种凝集素样相互作用介导的，其作用的发挥取决于寄生虫的表型以及宿主细胞上是否存在受体。
