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[不同来源组织中含表皮抑素复合物的免疫和电泳检测]

[Immunologic and electrophoretic detection of an epidermal chalone-containing complex in tissues of different origin].

作者信息

Ikulov V B, Ketlinskiĭ S A

出版信息

Arkh Anat Gistol Embriol. 1977 Feb;72(2):84-8.

PMID:140638
Abstract

The rabbit antiserum to one of the components of the tissue-specific protein complex isolated from the back skin of rats and containing G1- and G2-chalones was obtained. By means of this antiserum an antigen identical to the cutaneous one was found in the mucous membrane of the tongue, oesophagus, prestomach and vagina, in the epidermis of the tail and sole. The cornea, mucous membranes of the urinary bladder and intestine, liver, kidney and blood serum do not contain this antigen. According to data of disc-electrophoresis in 5%-polyacrilamide gel, the 55--81% alcohol extracts of the investigated tissues of the epidermal type, including the cornea and mucous membrane of the urinary bladder, are of similar quantitative and qualitative composition, different from that of tissues of other origin.

摘要

制备了针对从大鼠背部皮肤分离出的、含有G1和G2抑素的组织特异性蛋白质复合物的一种成分的兔抗血清。借助该抗血清,在舌、食管、前胃和阴道的黏膜、尾部和足底的表皮中发现了一种与皮肤抗原相同的抗原。角膜、膀胱和肠道的黏膜、肝脏、肾脏和血清中不含有这种抗原。根据在5%聚丙烯酰胺凝胶中进行圆盘电泳的数据,包括角膜和膀胱黏膜在内的表皮型被研究组织的55%-81%乙醇提取物具有相似的定量和定性组成,与其他来源的组织不同。

相似文献

1
[Immunologic and electrophoretic detection of an epidermal chalone-containing complex in tissues of different origin].[不同来源组织中含表皮抑素复合物的免疫和电泳检测]
Arkh Anat Gistol Embriol. 1977 Feb;72(2):84-8.
2
[Determination of epidermal G2-chalone in epidermal-type tissues].[表皮型组织中表皮G2抑素的测定]
Arkh Anat Gistol Embriol. 1982 Feb;82(2):100-6.
3
[Quantitative determination of the epidermal G2-chalonelike factor in rat tissues by means of radial immunodiffusion].[用放射免疫扩散法对大鼠组织中表皮G2类chalone因子进行定量测定]
Biull Eksp Biol Med. 1979 Jul;88(7):114-6.
4
[Purification and characterization of epidermal G2- and G1-chalones].[表皮G2期和G1期抑素的纯化与特性分析]
Biokhimiia. 1978 Jun;43(6):971-8.
5
[Immunologic determination of an epidermal G2-chalone-like factor as a marker of squamous cell structures in the urinary bladder and urine in rats].[大鼠膀胱和尿液中作为鳞状细胞结构标志物的表皮G2抑素样因子的免疫学测定]
Biull Eksp Biol Med. 1980 Dec;90(12):719-21.
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[Immunomorphological analysis of G2-chalone localization during the process of rat epidermis histogenesis].[大鼠表皮组织发生过程中G2抑素定位的免疫形态学分析]
Biull Eksp Biol Med. 1982 Jun;93(6):106-7.
7
[Immunological determination of epidermal G2-chalone as a squamous cell marker in lung tumors in rats].[大鼠肺肿瘤中作为鳞状细胞标志物的表皮G2抑素的免疫学测定]
Biull Eksp Biol Med. 1980 Aug;90(8):248-9.
8
Epidermal G1-chalone and transforming growth factor-beta are two different endogenous inhibitors of epidermal cell proliferation.表皮G1期抑素和转化生长因子-β是两种不同的表皮细胞增殖内源性抑制剂。
J Cell Physiol. 1990 Mar;142(3):496-504. doi: 10.1002/jcp.1041420308.
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[Use of immunochemical methods for characterization and purification of tissue-specific inhibitors of mitotic activity (chalones)].[利用免疫化学方法鉴定和纯化有丝分裂活性组织特异性抑制剂(抑素)]
Tsitologiia. 1975 Nov;17(11):1294-9.
10
[Detection of squamous cell differentiation in experimental tumors of urothelium by using epidermal G2 chalone].[利用表皮G2抑素检测尿路上皮实验性肿瘤中的鳞状细胞分化]
Arkh Patol. 1981;43(4):82-4.