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[不同来源组织中含表皮抑素复合物的免疫和电泳检测]

[Immunologic and electrophoretic detection of an epidermal chalone-containing complex in tissues of different origin].

作者信息

Ikulov V B, Ketlinskiĭ S A

出版信息

Arkh Anat Gistol Embriol. 1977 Feb;72(2):84-8.

PMID:140638
Abstract

The rabbit antiserum to one of the components of the tissue-specific protein complex isolated from the back skin of rats and containing G1- and G2-chalones was obtained. By means of this antiserum an antigen identical to the cutaneous one was found in the mucous membrane of the tongue, oesophagus, prestomach and vagina, in the epidermis of the tail and sole. The cornea, mucous membranes of the urinary bladder and intestine, liver, kidney and blood serum do not contain this antigen. According to data of disc-electrophoresis in 5%-polyacrilamide gel, the 55--81% alcohol extracts of the investigated tissues of the epidermal type, including the cornea and mucous membrane of the urinary bladder, are of similar quantitative and qualitative composition, different from that of tissues of other origin.

摘要

制备了针对从大鼠背部皮肤分离出的、含有G1和G2抑素的组织特异性蛋白质复合物的一种成分的兔抗血清。借助该抗血清,在舌、食管、前胃和阴道的黏膜、尾部和足底的表皮中发现了一种与皮肤抗原相同的抗原。角膜、膀胱和肠道的黏膜、肝脏、肾脏和血清中不含有这种抗原。根据在5%聚丙烯酰胺凝胶中进行圆盘电泳的数据,包括角膜和膀胱黏膜在内的表皮型被研究组织的55%-81%乙醇提取物具有相似的定量和定性组成,与其他来源的组织不同。

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