Jain S, Durand H, Tiraby G
Laboratoire de Microbiologie et Génétique Appliqués du C.N.R.S., C.R.B.G.C., Université Paul Sabatier, Toulouse, France.
Mol Gen Genet. 1992 Sep;234(3):489-93. doi: 10.1007/BF00538710.
A transformation system for the thermophilic cellulolytic fungus Talaromyces sp. CL240 has been developed, using the phleomycin resistance gene from Streptoalloteichus hindustanus (Sh ble) as a dominant selectable marker. The plasmids (pAN8-1 and pUT720) carrying the Sh ble gene under the control of the Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter, allowed selection of phleomycin-resistant transformants. A new promoter sequence cloned from chromosomal DNA of Trichoderma reesei (pUT737) was also able to drive efficient expression of the Sh ble gene in Talaromyces sp. CL240, resulting in the selection of transformants that were highly resistant to phleomycin.
已开发出一种用于嗜热纤维素分解真菌特异青霉属CL240的转化系统，该系统使用来自印度斯坦链霉菌（Sh ble）的博来霉素抗性基因作为显性选择标记。携带在构巢曲霉甘油醛-3-磷酸脱氢酶（gpd）启动子控制下的Sh ble基因的质粒（pAN8-1和pUT720），能够筛选出博来霉素抗性转化体。从里氏木霉染色体DNA克隆的一个新启动子序列（pUT737）也能够在特异青霉属CL240中驱动Sh ble基因的高效表达，从而筛选出对博来霉素高度抗性的转化体。
