HUBERT E G, KALMANSON G M, GUZE L B
J Bacteriol. 1963 Sep;86(3):569-72. doi: 10.1128/jb.86.3.569-572.1963.
Hubert, Earl G. (Veterans Administration Center, Los Angeles, Calif.), George M. Kalmanson, and Lucien B. Guze. Preservation of antigen-coated sheep erythrocytes by freezing for use in indirect hemagglutination procedure. J. Bacteriol. 86:569-572. 1963.-Preservation of sheep erythrocytes, coated with various protein and lipopolysaccharide antigens, was accomplished by rapid freezing and storage at -70 C in a dextrose-lactose solution. The stored cells were tested at intervals in an indirect hemagglutination procedure. Sensitivity of these cells was maintained, and antibody titers were consistently reproducible throughout the period of testing. These observations suggest that sheep erythrocytes may be coated with a variety of antigens and may be employed satisfactorily in an indirect hemagglutination test, even after storage at -70 C for a period up to 6 months.
休伯特，厄尔·G.（加利福尼亚州洛杉矶退伍军人管理中心），乔治·M.卡尔曼森，以及卢西恩·B.古兹。通过冷冻保存抗原包被的绵羊红细胞用于间接血凝试验。《细菌学杂志》86:569 - 572。1963年。——通过快速冷冻并在含葡萄糖 - 乳糖的溶液中于 -70℃保存，实现了用各种蛋白质和脂多糖抗原包被的绵羊红细胞的保存。在间接血凝试验中定期对保存的细胞进行检测。在整个检测期间，这些细胞的敏感性得以维持，抗体效价始终可重复。这些观察结果表明，绵羊红细胞可以包被多种抗原，并且即使在 -70℃保存长达6个月后，也可令人满意地用于间接血凝试验。
