Hiruma M, Kawada A, Noda T, Yamazaki M, Ishibashi A
Department of Dermatology, National Defense Medical College, Japan.
Mycoses. 1992 Jan-Feb;35(1-2):35-41. doi: 10.1111/j.1439-0507.1992.tb00816.x.
On the basis of electron microscopy examination of human tissue affected by sporotrichosis, we have proposed a classification, at the light microscopy level, of fungal cells in tissue. Light and electron microscopy observations clarified the following five points: (1) in sporotrichosis lesions in man, the fungal cells are frequently phagocytized by polymorphonuclear leucocytes (PMNs); (2) phagocytosis of phagocytes by other phagocytes was common, and giant cells phagocytized both fungal cells and PMNs; (3) fungal cells in the tissues of lesions were thought to be highly viable, and their budding was frequently observed within macrophages and giant cells; (4) it was highly probable that PMNs participated in asteroid body formation; (5) few free fungal cells were present in the tissues. We believe that the classification of fungal cells in sporotrichosis tissue into the five categories below is useful for correctly understanding the pathological condition in a sporotrichosis lesion: (1) fungal cells in PMNs, (2) fungal cells in PMNs within macrophages, (3) fungal cells in macrophages, (4) fungal cells in giant cells, and (5) free fungal cells.
基于对受孢子丝菌病影响的人体组织进行的电子显微镜检查，我们在光学显微镜水平上对组织中的真菌细胞提出了一种分类方法。光学显微镜和电子显微镜观察阐明了以下五点：（1）在人类孢子丝菌病病变中，真菌细胞常被多形核白细胞（PMN）吞噬；（2）吞噬细胞被其他吞噬细胞吞噬的情况很常见，巨细胞既吞噬真菌细胞也吞噬PMN；（3）病变组织中的真菌细胞被认为具有高度活力，且经常在巨噬细胞和巨细胞内观察到它们出芽；（4）PMN极有可能参与星状体的形成；（5）组织中存在的游离真菌细胞很少。我们认为，将孢子丝菌病组织中的真菌细胞分为以下五类，有助于正确理解孢子丝菌病病变中的病理状况：（1）PMN内的真菌细胞，（2）巨噬细胞内PMN中的真菌细胞，（3）巨噬细胞内的真菌细胞，（4）巨细胞内的真菌细胞，（5）游离真菌细胞。
